Thermoacoustic molecular imaging of small animals

被引：182

作者：

Kruger, Robert A. ^{[1
]}

Kiser, William L. ^{[1
]}

Reinecke, Daniel R. ^{[1
]}

Kruger, Gabe A. ^{[1
]}

Miller, Kathy D. ^{[1
]}

机构：

[1] OptoSonics, Inc., Indianapolis, IN 46268, 7210 Georgetown Road

来源：

Molecular Imaging | 2003年 / 2卷 / 02期

关键词：

Flourescence; Optical imaging; Optoacoustics; Photoacoustics; Thermoacoustics;

D O I：

10.1162/153535003322331993

中图分类号：

学科分类号：

摘要：

We have designed, constructed, and tested a thermoacoustic computed tomography (TCT) scanner for imaging optical absorption in small animals in three dimensions. The device utilizes pulsed laser irradiation (680-1064 nm) and a unique, 128-element transducer array. We quantified the isotropic spatial resolution of this scanner to be 0.35 mm. We describe a dual-wavelength substraction technique for isolating optical dyes with TCT. Phantom experiments demonstrate that we can detect 5 fmol of a near-infrared dye (indocyanine green, ICG) in a 1-μL volume using dual-wavelength subtraction. Initial TCT imaging in phantoms and in two sacrificed mice suggests that three-dimensional, optical absorption patterns in small animals can be detected with an order of magnitude better spatial resolution and an order of magnitude better low-contrast detectability in small animals when compared to fluorescence imaging or diffusion optical tomography.

引用

页码：113 / 123

页数：10

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