Cross talk between gibberellin and cytokinin: The Arabidopsis GA response inhibitor SPINDLY plays a positive role in cytokinin signaling

被引：260

作者：

Greenboim-Wainberg, Yaarit ^{[1
]}

Maymon, Inbar ^{[1
]}

Borochov, Roy ^{[1
]}

Alvarez, John ^{[2
]}

Olszewski, Neil ^{[3
]}

Ori, Naomi ^{[1
]}

Eshed, Yuval ^{[2
]}

Weiss, David ^{[1
]}

机构：

[1] Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem

[2] Department of Plant Sciences, Weizmann Institute of Science

[3] Department of Plant Biology, Plant Molecular Genetic Institute, University of Minnesota, St. Paul

来源：

Plant Cell | 2005年 / 17卷 / 01期

关键词：

D O I：

10.1105/tpc.104.028472

中图分类号：

学科分类号：

摘要：

SPINDLY (SPY) is a negative regulator of gibberellin (GA) responses; however, spy mutants exhibit various phenotypic alterations not found in GA-treated plants. Assaying for additional roles for SPY revealed that spy mutants are resistant to exogenously applied cytokinin. GA also repressed the effects of cytokinin, suggesting that there is cross talk between the two hormone-response pathways, which may involve SPY function. Two spy alleles showing severe (spy-4) and mild (spy-3) GA-associated phenotypes exhibited similar resistance to cytokinin, suggesting that SPY enhances cytokinin responses and inhibits GA signaling through distinct mechanisms. GA and spy repressed numerous cytokinin responses, from seedling development to senescence, indicating that cross talk occurs early in the cytokinin-signaling pathway. Because GA3 and spy-4 inhibited induction of the cytokinin primary-response gene, type-A Arabidopsis response regulator 5, SPY may interact with and modify elements from the phosphorelay cascade of the cytokinin signal transduction pathway. Cytokinin, on the other hand, had no effect on GA biosynthesis or responses. Our results demonstrate that SPY acts as both a repressor of GA responses and a positive regulator of cytokinin signaling. Hence, SPY may play a central role in the regulation of GA/cytokinin cross talk during plant development. © 2004 American Society of Plant Biologists.

引用

页码：92 / 102

页数：10

共 43 条

[1] Achard P., Vriezen W.H., Van Der Straeten D., Harberd N.P., Ethylene regulates Arabidopsis development via the modulation of DELLA protein growth repressor function, Plant Cell, 15, pp. 2816-2825, (2003)
[2] Arnon D.I., Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris, Plant Physiol., 24, pp. 1-15, (1949)
[3] Baum S.F., Eshed Y., Bowman J.L., The Arabidopsis nectary is an ABC-independent floral structure, Development, 128, pp. 4657-4667, (2001)
[4] Chen H., Banejrjee A.K., Hannapel D.J., The tandem complex of BEL and KNOX partners is required for transcriptional repression of ga20ox1, Plant J., 38, pp. 276-284, (2004)
[5] Chuck G., Lincoln C., Hake S., Knat1 induces lobed leaves with ectopic meristems when overexpressed in Arabidopsis, Plant Cell, 8, pp. 1277-1289, (1996)
[6] D'Agostino I.B., Deruere J., Kieber J.J., Characterization of the response of the Arabidopsis response regulator gene family to cytokinin, Plant Physiol., 124, pp. 1706-1717, (2000)
[7] Das A.K., Chohen P.T., Barford D., The structure of the tetratricopeptide repeats of protein phosphatase 5: Implication for TPR-mediated protein-protein interactions, EMBO J., 17, pp. 1192-1199, (1998)
[8] Ezura H., Harberd N.P., Endogenous gibberellin levels influence in-vitro shoot regeneration in Arabidopsis thaliana (L.) Heynh, Planta, 197, pp. 301-305, (1995)
[9] Filardo F.F., Swain S.M., SPYing on GA signaling and plant development, J. Plant Growth Regul., 22, pp. 163-175, (2003)
[10] Flick C.E., Evans D.E., Sharp W.R., Organogenesis, Handbook of Plant Cell Culture, 1, pp. 13-81, (1983)

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