In an attempt to understand the factors governing the aggregation of yeast in alcoholic fermentation, the influence of the medium composition was investigated. The surface physicochemical properties (hydrophobicity and electrical properties) and the surface chemical composition of the cells, measured by X-ray photoelectron spectroscopy, were evaluated concomitantly. Phosphate depletion in the culture medium led to a lower phosphate concentration at the cell surface; depletion of other nutrients resulted in an increase of phosphate surface concentration due to a lower amount of biomass. The electrical surface properties of the cells depended strongly on the phosphate surface concentration and influenced the tendency of the cells to aggregate. The aggregation of this yeast can be considered in most cases to be caused by the interactions between solvated macromolecules at the surface, the aggregates behaving like weak gels.