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Microbial population analysis as a measure of ecosystem restoration
被引：13
作者：
Haines, John R.
[1
]
Herrmann, Ronald
[1
]
Lee, Kenneth
[2
]
Cobanli, Susan
[2
]
Blaise, Christian
[3
]
机构：
[1] United States Environmental Protection Agency, 26 W. M.L. King Dr., Cincinnati, OH 45268, United States
[2] Bedford Institute of Oceanography, Dept. of Fisheries and Oceans, 1 Challenger Dr., Halifax, NS B2Y4A2, Canada
[3] Centre Saint-Laurent, Environment Canada, 105 McGill St., Montreal, QC H2Y2E7, Canada
关键词：
Phospholipids - Oil spills - Submarine geology - Hydrogen bonds - Aromatization - Ecosystems - Marine pollution - Polycyclic aromatic hydrocarbons - Paraffins - Sediments - Fatty acids - Nutrients - Toxicity;
D O I：
10.1080/10889860290777611
中图分类号：
学科分类号：
摘要：
During a controlled oil spill study in a freshwater wetland, four methods were used to track changes in microbial populations in response to in situ remediation treatments, including nutrient amendments and the removal of surface vegetation. Most probable number (MPN) estimates of alkane and aromatic hydrocarbon degraders showed divergence of the alkane and aromatic degrading populations during the first summer of the experiment. Alkane degraders increased in all plots by 1.5 orders of magnitude and aromatic degraders increased in oiled plots by 3.5 orders of magnitude. Phospholipid fatty acid (PLFA) analysis of biomass and community composition showed no essential differences among treatments. Denaturing gradient gel electrophoresis (DGGE) analysis of the sediment microbial community showed some differences in specific populations of organisms with respect to oiled and unoiled plots. Some organisms were only found in the oiled plots. Sediment toxicity measured against single celled algae showed that the oiled sediments were toxic into the second year of the study, but that nutrient addition relieved the toxicity more rapidly than natural attenuation of the oil. © 2002 by CRC Press LLC.
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页码：283 / 296
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