Application of microbial cells as multistep catalysts in potentiometric biosensing electrodes

被引：26

作者：

Kobos, R.K. ^{[1
]}

Pyon, H.Y. ^{[1
]}

机构：

[1] Department of Chemistry, Virginia Commonwealth University, Richmond,VA,23284, United States

来源：

Biotechnology and Bioengineering | 1981年 / 23卷 / 03期

关键词：

Chemical sensors - Electrodes - Potentiometers (electric measuring instruments);

D O I：

10.1002/bit.260230312

中图分类号：

学科分类号：

摘要：

The advantages of using intact microbial cells to mediate complex reaction sequences in the construction of biosensing electrodes are domonstrated. A potentiometric sensor was developed for nitrilotriacetic acid (NTA) in which a strain of Pseudomonas sp. was coupled to an ammonia gas-sensing electrode. the bacterial cells carry out a four-step reaction sequence to produce the measured species, ammonia, from NTA. The resulting microbial electrode responded to NTA in the concentration range of 1 × 10-4 M to 7 × 10-4M, with a slope of 35-40 mV/decade. The electrode was stable for up to one month. © 1981 John Wiley & Sons, Inc.

引用

页码：627 / 633