Diverse transcriptional initiation revealed by fine, large‐scale mapping of mRNA start sites

被引：132

作者：

Yutaka Suzuki ^{[1
]}

Hirotoshi Taira ^{[2
]}

Tatsuhiko Tsunoda ^{[3
]}

Junko Mizushima‐Sugano ^{[4
]}

Jun Sese ^{[2
]}

Hiroko Hata ^{[3
]}

Toshio Ota ^{[1
]}

Takao Isogai ^{[2
]}

Toshihiro Tanaka ^{[5
]}

Shinichi Morishita ^{[1
]}

Kousaku Okubo ^{[5
]}

Yoshiyuki Sakaki ^{[6
]}

Yusuke Nakamura ^{[6
]}

Akira Suyama ^{[5
]}

Sumio Sugano ^{[7
]}

机构：

[1] Department of Virology,

[2] Institute of Medical Science,undefined

[3] University of Tokyo,undefined

[4] Human Genome Center,undefined

[5] Institute of Medical Science,undefined

[6] University of Tokyo,undefined

[7] Genome Science Center,undefined

[8] Institute of Physical and Chemical Research (RIKEN),undefined

[9] Intelligent Communication Laboratory,undefined

[10] NTT Communication Science Laboratories,undefined

[11] Department of Complexity Science and Engineering Graduate School of Frontier Science,undefined

[12] University of Tokyo,undefined

[13] Helix Research Institute,undefined

[14] Institute of Molecular and Cell Biology,undefined

[15] University of Osaka,undefined

[16] Department of Life Sciences,undefined

[17] University of Tokyo,undefined

来源：

The EMBO Reports | 2001年 / 2卷 / 5期

关键词：

D O I：

10.1093/embo-reports/kve085

中图分类号：

学科分类号：

摘要：

Determination of the mRNA start site is the first step in identifying the promoter region, which is of key importance for transcriptional regulation of gene expression. The ‘oligo‐capping’ method enabled us to introduce a sequence tag to the first base of an mRNA by replacing the cap structure of the mRNA. Using cDNA libraries made from oligo‐capped mRNAs, we could identify the transcriptional start site of an individual mRNA just by sequencing the 5′‐end of the cDNA. The fine mapping of transcriptional start sites was performed for 5880 mRNAs in 276 human genes. Contrary to our expectations, the majority of the genes showed a diverse distribution of transcriptional start sites. They were distributed over 61.7 bp with a standard deviation of 19.5. Our finding may reflect the dynamic nature of transcriptional initiation events of human genes in vivo.

引用

页码：388 / 393

页数：5

共 75 条

[1] Altschul S.F.(1990)Basic local alignment search tool J. Mol. Biol. 215 403-410
[2] Gish W.(1977)Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease‐digested hybrids Cell 12 721-732
[3] Miller W.(1999)Yeast RNA polymerase II at 5 Å resolution Cell 98 799-810
[4] Myers E.W.(1999)Expanding the TRANSFAC database towards an expert system of regulatory molecular mechanisms Nucleic Acids Res. 27 318-322
[5] Lipman D.J.(1997)Analysis of exon/intron structure and 400 kb of genomic sequence surrounding the 5′‐promoter and 3′‐terminal ends of the human glypican 3 (GPC3) gene Genomics 45 48-58
[6] Berk A.J.(1996)CENSOR—a program for identification and elimination of repetitive elements from DNA sequences Comput. Chem. 20 119-122
[7] Sharp P.A.(1998)Regulation of gene expression by TBP‐associated proteins Genes Dev. 12 1398-1408
[8] Fu J.(1994)Oligo‐capping: a simple method to replace the cap structure of eucaryotic mRNAs with oligoribonucleotides Gene 138 171-174
[9] Gnatt A.L.(1982)Transcriptional control signals of a eukaryotic protein‐coding gene Science 217 316-324
[10] Bushnell D.A.(1989)Transcriptional regulation in mammalian cells by sequence‐specific DNA binding proteins Science 245 371-378

← 1 2 3 4 5 6 7 8 →