A chloroplast DegP2 protease performs the primary cleavage of the photodamaged D1 protein in plant photosystem II

被引:193
作者
Kirsten Haußühl [1 ]
Bertil Andersson [1 ]
Iwona Adamska [2 ]
机构
[1] Department of Biochemistry,
[2] Arrhenius Laboratories for Natural Sciences,undefined
[3] Stockholm University,undefined
[4] Division of Cell Biology,undefined
[5] Linköping University,undefined
关键词
D1 protein; DegP2 protease; non‐appressed thylakoids; photodamage; primary cleavage;
D O I
10.1093/emboj/20.4.713
中图分类号
学科分类号
摘要
Although light is the ultimate substrate in photosynthesis, it can also be harmful and lead to oxidative damage of the photosynthetic apparatus. The main target for light stress is the central oxygen‐evolving photosystem II (PSII) and its D1 reaction centre protein. Degradation of the damaged D1 protein and its rapid replacement by a de novo synthesized copy represent the important repair mechanism of PSII crucial for plant survival under light stress conditions. Here we report the isolation of a single‐copy nuclear gene from Arabidopsis thaliana, encoding a protease that performs GTP‐dependent primary cleavage of the photodamaged D1 protein and hence catalysing the key step in the repair cycle in plants. This protease, designated DegP2, is a homologue of the prokaryotic Deg/Htr family of serine endopeptidases and is associated with the stromal side of the non‐appressed region of the thylakoid membranes. Increased expression of DegP2 under high salt, desiccation and light stress conditions was measured at the protein level.
引用
收藏
页码:713 / 722
页数:9
相关论文
共 165 条
[1]  
Andersson B(1980)Lateral heterogeneity in the distribution of chlorophyll–protein complexes of the thylakoid membranes of spinach chloroplasts Biochim Biophys Acta 593 427-440
[2]  
Anderson JM(1984)Immunological studies on the organization of proteins in photosynthetic oxygen evolution Biochim Biophys Acta 766 21-28
[3]  
Andersson B(1993)Photoinhibition of photosystem II. Inactivation, protein damage and turnover Biochim Biophys Acta 1143 113-134
[4]  
Larsson C(1991)Breakdown of the photosystem II reaction center D1 protein under photoinhibitory conditions: identification and localization of the C‐terminal degradation products Biochemistry 30 10220-10226
[5]  
Jansson C(1992)Too much of a good thing: light can be bad for photosynthesis Trends Biochem Sci 17 61-66
[6]  
Ljungberg U(1999)Photosystem II Curr Opin Struct Biol 9 469-475
[7]  
Åkerlund H‐E(1993)Detection of a 10 kDa breakdown product containing the C‐terminus of the D1‐protein in photoinhibited wheat leaves suggests an acceptor side mechanism FEBS Lett 324 341-344
[8]  
Aro E‐M(1986)Import of proteins into chloroplasts. Membrane integration of a thylakoid precursor protein reconstituted in chloroplast lysates J Biol Chem 261 14804-14810
[9]  
Virgin I(1997)Prediction of transmembrane α‐helices in prokaryotic membrane proteins: the dense alignment surface method Protein Eng 10 673-676
[10]  
Andersson B(1998)β‐carotene to zeaxanthin conversion in the rapid turnover of the D1 protein of photosystem II FEBS Lett 424 267-270