PREPARATION OF MONOVALENT SUCCINYL-CONCANAVALIN A AND ITS MITOGENIC ACTIVITY

被引：18

作者：

BEPPU, M ^{[1
]}

TERAO, T ^{[1
]}

OSAWA, T ^{[1
]}

机构：

[1] UNIV TOKYO, FAC PHARM SCI, DIV CHEM TOXICOL & IMMUNOCHEM, BUNKYO KU, TOKYO 113, JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1976年 / 79卷 / 05期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a131152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monovalent succinyl-concanavalin A was prepared by photoaffinity labeling of succinyl-concanavalin A with p-azidophenyl .alpha.-D-mannopyranoside. This concanavalin A derivative showed a weak but definite mitogenic activity against mouse splenic lymphocytes and human peripheral lymphocytes, suggesting that direct receptor cross-linkage on the cell surface is not an obligatory prerequisite for the activation of lymphocytes.

引用

页码：1113 / 1117

页数：5

共 11 条

[1] PROTEIN-CARBOHYDRATE INTERACTION .6. ISOLATION OF CONCANAVALIN A BY SPECIFIC ADSORPTION ON CROSS-LINKED DEXTRAN GELS [J].

AGRAWAL, BBL ;

GOLDSTEIN, IJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 147 (02) :262-+

[2] PHOTOAFFINITY LABELING OF CONCANAVALIN-A - PREPARATION OF A CONCANAVALIN-A DERIVATIVE WITH REDUCED VALENCE [J].

BEPPU, M ;

TERAO, T ;

OSAWA, T .

JOURNAL OF BIOCHEMISTRY, 1975, 78 (05) :1013-1019

[3]

CUNNINGHAM BA, 1972, BIOCHEMISTRY-US, V11, P3233, DOI 10.1021/bi00767a016

[4] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[5] CONCANAVALIN A DERIVATIVES WITH ALTERED BIOLOGICAL-ACTIVITIES [J].

GUNTHER, GR ;

WANG, JL ;

YAHARA, I ;

CUNNINGHAM, BA ;

EDELMAN, GM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (04) :1012-1016

[6]

KAWAGUCHI T, 1974, J BIOL CHEM, V249, P2786

[7] PURIFICATION AND CHARACTERIZATION OF A CYTISUS-TYPE ANTI-H(O) PHYTOHEMAGGLUTININ FROM ULEX EUROPEUS SEEDS [J].

MATSUMOTO, I ;

OSAWA, T .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1970, 140 (02) :484-+

[8]

MOWERY DF, 1963, METHODS CARBOHYDRATE, V2, P328

[9] THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS [J].

SCATCHARD, G .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1949, 51 (04) :660-672

[10] PHYTOMITOGEN FROM WISTARIA-FLORIBUNDA SEEDS AND ITS INTERACTION WITH HUMAN PERIPHERAL LYMPHOCYTES [J].

TOYOSHIMA, S ;

AKIYAMA, Y ;

NAKANO, K ;

TONOMURA, A ;

OSAWA, T .

BIOCHEMISTRY, 1971, 10 (24) :4457-+

← 1 2 →