人外周血淋巴细胞化学发光测定的方法学研究

被引：5

作者：

张学军 ^{[1
]}

刘琏 ^{[1
,2
]}

机构：

[1] 安徽医科大学微生物学教研室

来源：

中华微生物学和免疫学杂志 | 1990年 / 10卷 / 03期

关键词：

淋巴细胞; 化学发光; ConA;

D O I：

暂无

中图分类号：

R446.1 [生物化学检验、临床检验];

学科分类号：

摘要：

人外周血淋巴细胞(PBLC)受ConA或PHA刺激后, 能产生短暂的、Luminol依赖性的化学发光(CL)反应。实验表明, 在有限稀释范围内, 淋巴细胞、ConA或PHA浓度越高, 淋巴细胞化学发光(Ly-CL)的强度越高, Ly-CL强度依赖于淋巴细胞、ConA或PHA浓度。Luminol在1×10-4M时, Ly-CL最强。5种细胞稀释液中, 以pH7.2～7.4、含1～3%小牛血清(CS)的HBSS用于Ly-CL测定最佳。新分离的淋巴细胞放置4℃ 22小时、22℃ 8小时或37℃ 2小时内, Ly-CL变化不显著。Ly-CL测定具有简便、快速、灵敏度高、重复性好等优点, 有可能成为研究淋巴细胞早期激活或功能测定的一种新手段。

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