ISOPRENALINE STIMULATION OF CYCLIC-AMP PRODUCTION BY ISOLATED CELLS FROM ADULT RAT MYOCARDIUM

被引：42

作者：

POWELL, T ^{[1
]}

TWIST, VW ^{[1
]}

机构：

[1] MIDDLESEX HOSP, SCH MED, DEPT PHYS APPL MED, LONDON W1P 6DB, ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1976年 / 72卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(76)80260-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When 1.25 .times. 10-6 mol/l isoprenaline is added to suspensions of adult rat myocardial cells in Ca-free bicarbonate buffer, there is a 1.6-fold increase above control of cyclic[c]AMP levels 30 s after drug addition. The phosphodiesterase inhibitor methylisobutylxanthine (MIX) at 0.5 mmol/l increases the control level of cAMP and also the level of stimulation to 3-fold. When MIX is present, Ca (1 mmol/l) has no effect on either control or stimulated cAMP; omission of Mg results in a lowering of control cAMP, with the levels after stimulation unchanged. Propranolol at 8.45 .times. 10-6 mol/l abolishes stimulation by isoprenaline.

引用

页码：1218 / 1225

页数：8

共 21 条

[1] HORMONE-SENSITIVE ADENYLYL CYCLASES - USEFUL MODELS FOR STUDYING HORMONE RECEPTOR FUNCTIONS IN CELL-FREE SYSTEMS [J].

BIRNBAUMER, L .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 300 (02) :129-158

[2]

BROWN BL, 1972, ADV CYCL NUCL RES<D>, V2, P25

[3] SIMPLE AND SENSITIVE SATURATION ASSAY METHOD FOR MEASUREMENT OF ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE [J].

BROWN, BL ;

ALBANO, JDM ;

EKINS, RP ;

SGHERZI, AM ;

TAMPION, W .

BIOCHEMICAL JOURNAL, 1971, 121 (03) :561-+

[4]

DRUMMOND GI, 1970, J BIOL CHEM, V245, P976

[5]

FARMER BB, 1975, FED PROC, V34, P332

[6]

GOULD RP, 1972, J PHYSIOL-LONDON, V225, pP16

[7]

JOHNSON RA, 1973, J BIOL CHEM, V248, P5114

[8] CONTRACTILE PROTEINS OF HEART [J].

KATZ, AM .

PHYSIOLOGICAL REVIEWS, 1970, 50 (01) :63-+

[9]

Kukovetz W R, 1975, Adv Cyclic Nucleotide Res, V5, P395

[10] BETA-ADRENERGIC-RECEPTOR AND ADENYLATE-CYCLASE [J].

LEFKOWITZ, RJ ;

LIMBIRD, LE ;

MUKHERJEE, C ;

CARON, MG .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 457 (01) :1-39

← 1 2 3 →