CLEAVAGE OF PLANT CHROMOSOMES BY RESTRICTION ENDONUCLEASES

被引：14

作者：

SUBRAHMANYAM, NC ^{[1
]}

GOULD, AR ^{[1
]}

DOY, CH ^{[1
]}

机构：

[1] AUSTRALIAN NATL UNIV, RES SCH BIOL SCI, DEPT GENET, CANBERRA 2601, ACT, AUSTRALIA

来源：

PLANT SCIENCE LETTERS | 1976年 / 6卷 / 04期

关键词：

D O I：

10.1016/0304-4211(76)90048-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cytological observations were made on the root-tip cells of Hordeum vulgare and H. bulbosum treated with a site-specific endonuclease preparation (restriction enzymes Hind II and III from Haemophilus influenzae). The exogenously supplied endonuclease had cleaved the chromosomes in intact cells of both species into discrete fragments. Degradation was progressive with time. The number of breaks per H. vulgare chromosome was greater than per H. bulbosum chromosome. The possible implications of such differential susceptibility and practical applications of this technique are discussed.

引用

页码：203 / 208

页数：6

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