CHARACTERIZATION OF ONE NEUTRAL AND 2 ACIDIC GLYCOASPARAGINES ISOLATED FROM URINE OF PATIENTS WITH ASPARTYLGLYCOSYLAMINURIA (AGU)

被引：26

作者：

SUGAHARA, K

FUNAKOSHI, S

FUNAKOSHI, I

AULA, P

YAMASHINA, I

机构：

[1] KYOTO UNIV, FAC PHARM SCI, DEPT BIOL CHEM, SAKYO KU, KYOTO 606, JAPAN

[2] UNIV HELSINKI, DEPT PATHOL, SF-00290 HELSINKI, FINLAND

来源：

JOURNAL OF BIOCHEMISTRY | 1976年 / 80卷 / 02期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a131264

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The neutral glycoasparagine was identified as .beta.-Gal-(1 .fwdarw. 4)-.beta.-GlcNAc-Asn. The acidic ones were composed of 1 mol of sialic acid and 2 mol each of galactose and N-acetylglucosamine, attached to asparagine, and were isomeric with respect to the position of sialic acid attachment since they produced the same glycoasparagine on incubation with the neuraminidase [EC 3.2.1.18] from Clostridium perfringens. The structure of the resulting sialic acid-free glycoasparagine was determined to be .beta.-Gal-.beta.-GLcNAc-.beta.-Gal-(1 .fwdarw. 4)-.beta.-GlcNAc-Asn based mainly on the results of sequential enzymatic degradations.

引用

页码：195 / 201

页数：7

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