Synergistic Interactions of a Synthetic Lubricin-Mimetic with Fibronectin for Enhanced Wear Protection

被引：9

作者：

Andresen Eguiluz R.C. ^{[1
,4
,5
]}

Cook S.G. ^{[1
]}

Tan M. ^{[2
,4
,5
]}

Brown C.N. ^{[1
]}

Pacifici N.J. ^{[1
]}

Samak M.S. ^{[3
]}

Bonassar L.J. ^{[2
]}

Putnam D. ^{[2
]}

Gourdon D. ^{[1
,3
]}

机构：

[1] Department of Materials Science and Engineering, Cornell University, Ithaca, NY

[2] Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY

[3] Department of Physics, University of Ottawa, Ottawa, ON

[4] Roberto C. Andresen Eguiluz, Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL

[5] The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA

来源：

Frontiers in Bioengineering and Biotechnology | 2017年 / 5卷

基金：

加拿大自然科学与工程研究理事会; 美国国家卫生研究院;

关键词：

bottlebrush polymer; fibronectin; lubricin-mimetic; surface forces apparatus; wear protection;

D O I：

10.3389/fbioe.2017.00036

中图分类号：

学科分类号：

摘要：

Lubricin (LUB), a major mucinous glycoprotein of mammalian synovial fluids, is believed to provide excellent lubrication to cartilage surfaces. Consequently, when joint disease or replacement leads to increased friction and surface damage in the joint, robust synthetic LUB alternatives that could be used therapeutically to improve lubrication and surface protection are needed. Here, we report the characterization of a lubricating multiblock bottlebrush polymer whose architecture was inspired by LUB, and we investigate the role of fibronectin (FN), a glycoprotein found in the superficial zone of cartilage, in mediating the tribological properties of the polymer upon shear between mica surfaces. Our surface forces apparatus (SFA) normal force measurements indicate that the lubricin-mimetic (mimLUB) could be kept anchored between mica surfaces, even under high contact pressures, when an intermediate layer of FN was present. Additional SFA friction measurements show that FN would also extend the wearless friction regime of the polymer up to pressures of 3.4 MPa while ensuring stable friction coefficients (μ ≈ 0.28). These results demonstrate synergistic interactions between mimLUB and FN in assisting the lubrication and wear protection of ideal (mica) substrates upon shear. Collectively, these findings suggest that our proposed mimLUB might be a promising alternative to LUB, as similar mechanisms could potentially facilitate the interaction between the polymer and cartilage surfaces in articular joints and prosthetic implants in vivo. © Copyright © 2017 Andresen Eguiluz, Cook, Tan, Brown, Pacifici, Samak, Bonassar, Putnam and Gourdon.

引用

共 52 条

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