用绿色荧光蛋白标记的细菌研究鱼体吸收颗粒抗原的部位

被引：3

作者：

陈营

陆承平

机构：

[1] 南京农业大学农业部动物疫病诊断与免疫重点开放实验室!江苏南京

来源：

水产学报 | 2000年 / 05期

关键词：

抗原吸收; 绿色荧光蛋白; 嗜水气单胞菌; 异育银鲫;

D O I：

暂无

中图分类号：

S942.5 [人工免疫];

学科分类号：

0906 ;

摘要：

用绿色荧光蛋白标记的嗜水气单胞菌 4332株 (Ah4332 GFP)菌液高渗浸泡和直接浸泡异育银鲫 ,研究鱼体对颗粒抗原的吸收。经两种方法处理的鱼 ,在整个实验过程中显示 ,鱼鳃的标记菌检出量均高于皮肤和肠道中的标记菌 ,证实鳃是抗原吸收的主要部位。另外 ,鱼体经高渗处理 ,鳃和皮肤可检出的标记菌明显增加。在肠道中 ,除浸泡 6 0min外 ,浸泡 10min、30min及 12 0min的细菌检出量也明显增加。显示高渗可促进抗原的吸收

引用

页码：472 / 475

页数：4

共 13 条

[1]

Green fluorescent protein as a reporter of gene expression and protein localization. Kain S R,Adams M,Kondepudi A. Biotechniques . 1995

[2]

Monitoring of GFP-Tagged Bacterial cells. Tombolini R,Jansson J K. Methods in Molecular Biology . 1998

[3]

Electron microscopic examination of antigen uptake by salmonid gill cells after bath immunization with a bacterin. Zapata A G,Torroba M,Alvarez F. Journal of Fish Biology . 1987

[4]

Particulate antigen uptake during immersion immunisation of fish: The effectiveness of prolonged exposure and the roles of skin and gill. Moore J D,Ototake M,Nakanishi T. Fish and Shellfish Immunology . 1998

[5]

The uptake of bovine serum by the skin of bath-immunised rainbow trout Oncorhynchus mykiss. Ototake M,Iwama G K,Nakanishi T. Fish and Shellfish Immunology . 1996

[6]

Uptake of bovin serum albumin by rainbow trout from hyperosmotic infiltration: a model for vaccinating fish. Amend D F,Fender D C. Science . 1976

[7]

Hyperosmotic infiltration of bacteria into trout: Route of entry and the fate of infiltrated bacteria. Alexander J B,Bowers A,Shamshoon S M. Developments in Biological Standardization . 1981

[8]

Hyperosmotic infiltration: Immunological demonstration of infiltrating bacteria in brown trout, Salmo Trutta L. Bowers A,Alexander J B. Journal of Fish Biology . 1981

[9]

Location of Pasteurella piscicida antigen in tissue of yellowtail Seriola quinqueradiata vaccinated by immersion. Kawahara E,Kusuda R. Jap Fish Sci Soc . 1981

[10]

Analysis of the hyperosmotic and bath methods for fish vaccination comparison of uptake of particulate and non-particulate antigens. Smith P D. Development and Comparative Immunology . 1982

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