HMGB1与claudin-1在重症急性胰腺炎肠黏膜屏障损伤中的表达及意义

被引：3

作者：

栾正刚 ^{[1
]}

郭仁宣 ^{[2
]}

机构：

[1] 中国医科大学附属第一医院重症医学科

[2] 中国医科大学附属第一医院普通外科

来源：

中国普通外科杂志 | 2013年 / 22卷 / 03期

关键词：

胰腺炎,急性坏死性; 肠黏膜; HMGB1蛋白质; 连接蛋白类;

D O I：

暂无

中图分类号：

R657.51 [];

学科分类号：

摘要：

目的:探讨高迁移率族蛋白B1(HMGB1)与claudin-1在重症急性胰腺炎(SAP)肠黏膜屏障损伤时的表达改变及意义。方法:将24只雄性Wistar大鼠随机均分为假手术组,SAP模型组(模型组),SAP模型+HMGB1抑制剂丙酮酸乙酯(EP)处理组(EP处理组)。SAP模型采用5%牛磺胆胆酸钠逆行胰管注射法,EP处理组于造模后2 h尾静脉注射EP溶液。各组大鼠于术后24 h处死,测定血浆淀粉酶(AMY),内毒素(LPS),血浆二胺氧化酶(DAO)水平;观察肠黏膜的病理学变化;免疫组织化学法观察肠黏膜claudin-1蛋白的表达;RT-PCR法检测肠组织HMGB1和claudin-1 mRNA的表达。结果:与假手术组比较,模型组大鼠血浆AMY,LPS和DAO水平均明显升高(均P<0.05);小肠黏膜屏障受损,上皮细胞间紧密连接破坏;肠黏膜claudin-1蛋白表达降低,肠组织HMGB1mRNA表达升高,claudin-1 mRNA的表达降低。与模型组比较,EP处理组肠组织HMGB1 mRNA表达降低,claudin-1蛋白与mRNA表达升高,各损伤指标均明显改善。结论:SAP肠黏膜屏障损伤可能与肠组织HMGB1表达升高,从而下调紧密连接蛋白claudin-1的表达有关;HMGB1抑制剂EP对SAP肠黏膜屏障损伤具有保护作用。

引用

页码：265 / 269

页数：5

共 10 条

[1] 重症急性胰腺炎肠黏膜屏障功能障碍细菌易位的研究现状
李振华
王湘英
[J]. 中国普通外科杂志, 2011, 20 (03) : 286 - 291
[2] 清胰颗粒对重症急性胰腺炎大鼠回肠claudin-1表达的影响
张淑坤
崔乃强
赵光
沈银丰
魏巍
[J]. 中国普通外科杂志, 2010, 19 (09) : 980 - 983
[3] Activation of phosphorylating-p38 mitogen-activated protein kinase and its relationship with localization of intestinal stem cells in rats after ischemia-reperfusion injury[J]. Xiao-Bing Fu Feng Xing Yin-Hui Yang Tong-Zhu Sun Bao-Chen Guo Wound Healing and Cell Biology Laboratory,Institute of Burns,304 Hospital,Trauma Center of Postgraduate Medical College,Beijing 100037,China. World Journal of Gastroenterology. 2003(09)
[4] Surgical Management of Late Sequelae in Survivors of an Episode of Acute Necrotizing Pancreatitis
Beck, William C.
Bhutani, Manoop S.
Raju, Gottumukkala S.
Nealon, William H.
[J]. JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS, 2012, 214 (04) : 682 - 688
[5] Ethyl pyruvate: a novel anti‐inflammatory agent[J] . M. P.Fink. Journal of Internal Medicine . 2007 (4)
[6] Factors Influencing Mortality in Acute Pancreatitis: Can We Alter Them?[J] . C S Pitchumoni,Nayan M Patel,Prasanna Shah. Journal of Clinical Gastroenterology . 2005 (9)
[7] Ethyl pyruvate ameliorates distant organ injury in a murine model of acute necrotizing pancreatitis
Yang, RK
Uchiyama, T
Alber, SM
Han, XN
Watkins, SK
Delude, RL
Fink, MP
[J]. CRITICAL CARE MEDICINE, 2004, 32 (07) : 1453 - 1459
[8] Extracellular role of HMGB1 in inflammation and sepsis
Wang, H
Yang, H
Tracey, KJ
[J]. JOURNAL OF INTERNAL MEDICINE, 2004, 255 (03) : 320 - 331
[9] Bacterial translocation and its prevention in acute pancreatitis[J] . Christos Dervenis,Dimitrios Smailis,Efthimios Hatzitheoklitos. Journal of Hepato-Biliary-Pancreatic Surgery . 2003 (6)
[10] Evaluation of factors that have reduced mortality from acute pancreatitis over the past 20 years
Bank, S
Singh, P
Pooran, N
Stark, B
[J]. JOURNAL OF CLINICAL GASTROENTEROLOGY, 2002, 35 (01) : 50 - 60

← 1 →