黄芪三萜皂苷通过抑制TLR-4/NF-κB p65信号调节的炎症反应而发挥保护病毒性心肌炎的作用研究

被引：11

作者：

杨怡侠 ^{[1
]}

刘天龙 ^{[2
]}

刘晶 ^{[2
]}

张勇 ^{[1
]}

杨秀华 ^{[3
]}

肖云峰 ^{[3
]}

刘小雷 ^{[3
]}

机构：

[1] 呼和浩特市蒙医中医医院药剂科

[2] 内蒙古医科大学附属医院药剂科

[3] 内蒙古医科大学药理学教研室

来源：

中南药学 | 2018年 / 16卷 / 12期

关键词：

黄芪三萜皂苷; 病毒性心肌炎; 炎症; TLR-4/NF-κB p65信号;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的研究黄芪三萜皂苷(AST)对病毒性心肌炎的治疗作用及机制。方法利用CVB3病毒和BALB/c小鼠制备病毒性心肌炎模型,并使用AST进行干预。记录各组生存曲线,行心肌组织HE染色,检测血浆中心肌肌钙蛋白T(c Tn T)水平及心肌组织中ANP、BNP、IL-1β、IL-6、TNF-α及INF-γ表达水平以评价心肌的炎症水平,检测心肌组织中TLR-4、Phospho-p65-NF-κB表达和活化水平以探讨AST抗CVB3诱导炎症的机制。结果 AST能够显著提高小鼠的生存率,减少小鼠CVB3诱导的心肌损伤面积,降低血液中c Tn T水平,抑制CVB3诱导的ANP和BNP在心肌组织中表达。AST对CVB3诱导小鼠心肌组织中IL-1β、IL-6、TNF-α及INF-γ表达水平增加有显著的抑制作用。CVB3诱导组小鼠心肌组织中TLR-4表达水平显著增加,磷酸化NF-κB p65表达和活性均显著增加,而AST对CVB3诱导的TLR-4/NF-κB p65信号的活化具有显著抑制作用。结论 AST对CVB3诱导的病毒性心肌炎具有显著的治疗作用,主要是通过抑制CVB3诱导的TLR-4/NF-κB p65信号活化,减弱心肌组织的炎症反应实现的。

引用

页码：1705 / 1709

页数：5

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