余甘子中水溶性鞣质的抗动脉粥样硬化作用机制研究

被引：10

作者：

呙爱秀

黄兴国

唐湘云

机构：

[1] 湖南环境生物职业技术学院

来源：

实用预防医学 | 2007年 / 02期

关键词：

血管内皮细胞; 血管平滑肌细胞; Phy-13; Phy-16; 氧化损伤; 增殖;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

1008 ;

摘要：

目的在细胞水平证实中药余甘子(Phyllanthus Emblica L.)的两个可溶性鞣质成分Phy-13(beta-1-O-galloyl-3,6-(R)-hexahydroxydiphenoyl-d-glucose)和Phy-16(1,6-di-O-galloyl-beta-d-glucose)是否具有抗动脉粥样硬化作用。方法人脐动脉内皮细胞ECV-304经ox-LDL(50 mg/l)诱导,MDA试剂盒检测Phy-13和Phy-16作用后内皮细胞培养上清液中丙二醛(MDA)的水平,MTT法检测动脉血管平滑肌细胞(VSMC)的增殖,细胞计数仪计数单核细胞和内皮细胞的黏附。结果Phy-13和Phy-16能减少内皮细胞MDA的生成,减少ox-LDL诱发的单核细胞和内皮细胞黏附,并能对抗ox-LDL诱导的动脉血管平滑肌细胞(VSMC)增殖。结论Phy-13和Phy-16能够对抗ox-LDL诱导的动脉粥样硬化,这可能是余甘子治疗动脉粥样硬化相关疾病的重要机制之一。

引用

页码：352 / 355

页数：4

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