SARS冠状病毒PUMC01分离株的基因组序列分析

被引:6
作者
邹柯
朱华
丁克越
王仲
刘勇
王婷
杨剑
魏国柱
周鑫峰
张文
于占霞
樊峥
彭晓忠
秦川
刘湘军
沈岩
倪安平
强伯勤
机构
[1] NationalLaboratoryofMedicalMolecularBiology,InstituteofBasicMedicalSciences,CAMSandPUMC,Beijing,InstituteofExperimentalAnimal,CAMSandPUMC,Beijing,NationalLaboratoryofMedicalMolecularBiology,InstituteofBasicMedicalSciences,CAMSandPUMC,Beijing,PUMCHospital,C
关键词
严重急性呼吸综合征; 冠状病毒; 基因组;
D O I
暂无
中图分类号
R373 [人体病毒学(致病病毒)];
学科分类号
摘要
目的对北京协和医院分离的SARS冠状病毒(SARS-CoV)PUMC01株的基因组序列进行变异及系统发育分析。方法利用随机引物法构建SARS-CoV-PUMC01分离株的cDNA文库,随机挑选质粒克隆进行大规模测序,测序反应经过组装后获得全基因组序列(GenbankAccessionNo.,AY350750),对该序列与SARS-CoV的参考序列相比进行系统发育及变异分析。结果与参考序列(SARS-CoV-Tor2及SARS-CoV-Urbani)相比较,在SARS-CoV-PUMC01上共发现10个变异位点;与其他的17株SARS-CoV进行系统发育分析后发现,18株SARS-CoV分为两类,两类之间及每一类的各株病毒间具有不同的分化时间。结论了解不同地区SARS-CoV之间的系统发育关系,为系统了解不同SARS-CoV分离株之间的临床关系以及SARS-CoV的传播链提供了进化上的证据。
引用
收藏
页码:495 / 498
页数:4
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