高温诱导体外培养奶牛乳腺上皮细胞的应激响应

被引：46

作者：

胡菡 ^{[1
,2
]}

王加启 ^{[1
,2
]}

李发弟 ^{[2
]}

卜登攀 ^{[2
]}

周凌云 ^{[2
]}

崔瑞莲 ^{[2
]}

机构：

[1] 甘肃农业大学动物科学技术学院

[2] 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室

来源：

农业生物技术学报 | 2011年 / 19卷 / 02期

关键词：

奶牛乳腺上皮细胞; 热应激; 热休克蛋白; 酪蛋白; 乙酰辅酶A羧化酶; 嗜乳脂蛋白; 脂肪酸;

D O I：

暂无

中图分类号：

S823 [牛];

学科分类号：

090502 [动物营养与饲料科学];

摘要：

实验以体外培养的奶牛乳腺上皮细胞为模型,研究不同时间高温(42℃)热处理对细胞热休克蛋白HSP、细胞凋亡、乳脂肪和乳蛋白合成相关基因mRNA表达丰度的影响。结果发现,热休克转录子hsf-1和热休克蛋白基因hsp27、hsp70和hsp90在高温处理0.5h后mRNA表达上调,其中hsp70转录水平有急剧上调过程,且随热处理时间延长均表现出先上升后下降的趋势;标志细胞凋亡的Bax基因从处理的0.5h开始mRNA表达下调,随后升高;但细胞凋亡Bcl-2mRNA表达上调;在检测的热处理过程中,αS1-酪蛋白(CSN1S1)基因转录先上调后下降,而β-酪蛋白(CSN2)和嗜乳脂蛋白(BTN)基因转录均下调;与脂肪酸合成和调控相关的基因乙酰辅酶A羧化酶(ACACA)、过氧化物酶激活受体-γ(PPARG)和固醇调节元件转录因子-1(SREBF-1)基因表达显著上调,而过氧化物酶激活受体-α(PPARA)基因mRNA转录没有明显变化。本实验结果证明热应激诱导了奶牛乳腺上皮细胞的应激反应,对细胞的乳蛋白和乳脂肪合成功能产生了显著影响,细胞产生热耐受。

引用

页码：287 / 293

页数：7

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