麦冬皂苷B对A549细胞株体外黏附、侵袭及迁移的抑制作用及机制研究

被引:27
作者
陈美娟
赵若琳
郭园园
宁利敏
张旭
机构
[1] 南京中医药大学江苏省中医药防治肿瘤协同创新中心
关键词
麦冬皂苷B; 非小细胞肺癌; 黏附; 侵袭; 迁移; 基质金属蛋白酶-2; 基质金属蛋白酶-9;
D O I
暂无
中图分类号
R285 [中药药理学];
学科分类号
100806 [中药药理学];
摘要
目的探究麦冬皂苷B(ophiopogonin-B,OP-B)抑制非小细胞肺癌细胞株A549黏附、侵袭、迁移的作用及其机制。方法细胞黏附实验、Transwell小室实验分别检测细胞黏附、迁移和侵袭能力。通过荧光实时定量反转录PCR(qRT-PCR)检测OP-B对A549细胞中MMP-2/9 mRNA表达水平的调控,Western blot法检测细胞中MMP-2/9蛋白及其上游调控蛋白Akt及其磷酸化水平的变化。结果 10μmol·L-1浓度的OP-B能明显抑制A549细胞黏附、侵袭和迁移,同时能抑制其MMP-2/9 mRNA及蛋白水平的表达,并抑制其上游p-Akt的表达。结论 OP-B有效抑制A549细胞黏附、侵袭和迁移,其作用与下调MMP-2/9 mRNA和蛋白水平的表达及抑制其上游Akt的磷酸化有关。
引用
收藏
页码:660 / 664
页数:5
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