久效磷对金鱼精巢超显微结构和特征性酶活性的影响

被引:4
作者
房燕
汝少国
邴欣
姜明
机构
[1] 中国海洋大学海洋生命学院
关键词
金鱼; 久效磷; 乳酸脱氢酶; 磷酸酶; 超微结构; 精巢;
D O I
暂无
中图分类号
X503.225 [鱼类、水产];
学科分类号
摘要
用0.01、0.10、1.00 mg.L-1久效磷暴露金鱼21 d,测定了精巢乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、碱性磷酸酶(ALP)的活性,并观察了精巢显微和超微结构的变化。结果表明:久效磷暴露21 d,精巢小叶基膜断裂,Leydig氏细胞水肿,小叶间质扩大,支持细胞核膜溶解,胞质内脂滴和髓磷脂象数量增多。1.00 mg.L-1久效磷造成了生殖腺指数的显著下降,对精巢发育有一定程度的延迟作用。随着久效磷暴露浓度的升高,LDH,ACP,ALP的活性逐渐下降,0.10、1.00 mg.L-1久效磷暴露组精巢LDH,ACP的活性下降显著,而各暴露组的精巢ALP活性呈现不显著下降趋势。推测久效磷可能通过损伤精巢超显微结构和降低LDH,ACP和ALP活性,干扰精巢能量代谢,造成对雄性金鱼的生殖毒性。
引用
收藏
页码:568 / 574
页数:7
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