依达拉奉对Aβ1-40诱导PC12细胞NF-κB、Bcl-2mRNA和蛋白表达的影响

被引：4

作者：

王鹏军

宋荣蓉

尚宏

机构：

[1] 哈尔滨医科大学附属第四医院神经内科

来源：

中国老年学杂志 | 2009年 / 29卷 / 18期

关键词：

依达拉奉; NF-κB; Bcl-2; 阿尔茨海默病; 细胞凋亡;

D O I：

暂无

中图分类号：

R749.16 [];

学科分类号：

100203 ;

摘要：

目的探讨依达拉奉对β淀粉样蛋白(Aβ1-40)诱导PC12细胞NF-κB、Bcl-2 mRNA和蛋白表达的影响。方法采用Western印迹法,RT-PCR等检测NF-κB、Bcl-2 mRNA和蛋白的表达,观察MCI-186对其保护作用。结果模型组中NF-κBP65 mRNA和蛋白表达水平明显升高,Bcl-2 mRNA和蛋白表达水平明显降低,与对照组比较有显著差异(P<0.01)。保护组中NF-κBP65 mRNA和蛋白及Bcl-2 mRNA和蛋白表达与模型组组间比较有统计学意义(均P<0.05),而与对照组比较无统计学意义(P>0.05)。结论Aβ1-40可以活化神经细胞内NF-κB,减少Bcl-2的表达,发生细胞凋亡。依达拉奉可以抑制NF-κB激活,增加Bcl-2的表达发挥抗凋亡作用,最终达到保护神经细胞的目的。

引用

页码：2360 / 2362

页数：3

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