低氧训练对大鼠骨骼肌HIF-1α基因表达的影响

被引：3

作者：

赵鹏 ^{[1
]}

路瑛丽 ^{[1
]}

冯连世 ^{[1
]}

徐建方 ^{[1
]}

朱珂 ^{[2
]}

机构：

[1] 国家体育总局体育科学研究所

[2] 河南省体育科学研究所

来源：

山东大学学报(理学版) | 2009年 / 44卷 / 05期

关键词：

低氧训练; HIF-1α; 基因表达; 骨骼肌;

D O I：

暂无

中图分类号：

R87 [运动医学];

学科分类号：

100216 ;

摘要：

探讨不同低氧训练模式对机体骨骼肌HIF-1αmRNA水平和蛋白水平表达的影响。6周龄SD雄性大鼠120只,经3周适应性训练和力竭实验筛选出90只,随机分成9组:常氧安静对照组、持续低氧安静组、间歇低氧安静组、低住低练耐力组、高住高练耐力组、高住低练耐力组、低住高练耐力组、高住高练后复氧训练组和高住低练后复氧训练组。采用常压低氧仓在13.6%的氧体积分数下(相当于海拔3 500 m的氧体积分数)进行低氧训练,根据血乳酸-速度曲线确定大鼠常氧训练的强度为35 m/min,低氧训练的强度为30 m/min。低氧训练持续时间为6周,每周训练5 d。其中,在第4周末进行运动能力测试,第5周末进行力竭测试,在第6周末的最后一次运动后休息48 h后处死,取材。采用实时荧光定量PCR、免疫组化、Western blot等技术测试大鼠骨骼肌HIF-1αmRNA水平和蛋白水平表达水平的变化,以进一步探讨低氧训练对骨骼肌HIF-1α表达的适应机制。结果显示,与低住低练组相比,高住高练组和高住低练骨骼肌HIF-1αmRNA表达有显著性升高(P<0.05);和常氧安静对照组相比,高住高练骨骼肌HIF-1αmRNA表达升高105%,有非常显著性差异(P<0.01);高住高练后复氧训练1周,大鼠骨骼肌HIF-1αmRNA表达有非常显著性降低(P<0.01),回到常氧安静对照组水平;高住低练后复氧训练1周,大鼠骨骼肌HIF-1αmRNA表达有显著性降低(P<0.05),回到常氧安静对照组水平。可得结论:高住高练、高住低练和持续低氧安静组骨骼肌HIF-1α表达都明显增强,而低住低练和低住高练变化不大,复氧训练后回到常氧安静水平。HIF-1α表达与低氧的程度和时间有明显的依存关系。

引用

页码：1 / 9

页数：9

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