大环内酯类药物对铜绿假单胞菌生物膜形成的影响

被引:9
作者
彭程
肖永红
机构
[1] 重庆医科大学附属第一医院!重庆
关键词
大环内酯类药物; 铜绿假单胞菌; 生物膜;
D O I
10.13461/j.cnki.cja.002957
中图分类号
R978.1 [抗生素];
学科分类号
100705 ;
摘要
采用聚碳酸酯过滤膜或聚丙烯酰胺膜制备铜绿假单胞菌生物膜 ,计数滤膜菌落并用扫描电镜观察 ,研究大环内酯类及抗铜绿假单胞菌药物对铜绿假单胞菌生物膜形成的影响。结果表明与未经药物作用的滤膜菌落计数比较 ,阿奇霉素 2 mg/ L 和红霉素 2 mg/ L 可使生物膜内细菌由 10 1 0 CFU/ cm2降至 10 3.3和 10 3.7CFU/ cm2 ;阿奇霉素 (2 mg/ L )分别与头孢他啶 (0 .0 6 2 5 mg/ L )、亚胺培南 (0 .5 mg/ L )、美洛培南 (0 .2 5 mg/ L )、阿米卡星 (0 .0 6 2 5 mg/ L )、环丙沙星 (0 .0 0 4mg/ L )合用后 ,生物膜内细菌降至 10 1 .5~ 10 3.8CFU / cm2 ;红霉素与上述药物合用后 ,生物膜内细菌降至 10 2 .8~ 10 5.5CFU/ cm2 ;麦迪霉素 32 mg/ L、抗铜绿假单胞菌药物单用或两者联合应用 ,菌量为 10 8.1~ 10 1 1 .5CFU/ cm2。扫描电镜观察与菌落计数结果一致。表明红霉素、阿奇霉素能抑制铜绿假单胞菌生物膜的形成 ;麦迪霉素及五种抗铜绿假单胞菌药物对生物膜的形成无抑制作用
引用
收藏
页码:286 / 289
页数:4
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