SELDI-TOF-MS技术在肝癌诊断中的临床应用性研究

被引：4

作者：

刘池波

梁勇

潘春琴

孙灵芬

金茜

机构：

[1] 台州市立医院

来源：

中国热带医学 | 2007年 / 01期

关键词：

表面增强激光解析电离飞行时间质谱; 蛋白质芯片; 肝癌;

D O I：

暂无

中图分类号：

R735.7 [肝肿瘤];

学科分类号：

摘要：

目的应用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)筛选肝癌患者血清标志蛋白,探讨作为临床诊断指标的最佳标志蛋白组合模式。方法对40例肝癌、20例肝硬化和60例健康志愿者的血清进行分析,并反复分析寻找合理运算规则以确定能够区分肝癌和健康志愿者与肝硬化患者的蛋白组学图谱。结果肝癌、肝硬化患者与健康对照组血清蛋白质指纹图谱之间有5个标志蛋白在肝癌患者血清中高表达,1个标志蛋白在肝癌患者血清中低表达。其中2个蛋白质峰[8943 Da和4477 Da质荷比(m/z)]组合构建的诊断模型Ⅰ鉴别肝癌和健康志愿者,灵敏度为90％(36/40),特异性为100％(60/60)。3个蛋白质峰(4477Da、13761Da和4097Da m/z)组合的诊断模型Ⅱ鉴别肝癌组与肝硬化组的灵敏度为85％(34/40),特异性为90％(18/20)。结论SELDI-TOF-MS技术的特异性及敏感度远远高于目前所采用的某一单独的标志物的血清学诊断,其结果对进一步研究肝癌的蛋白质组学改变及其临床应用可能具有重要意义。

引用

页码：18 / 20

页数：3

共 10 条

[1] PetrieoinⅢ,proteomic patterns of nipple aspirate fluids obtained by SELDI-TOF:potential for new biomarkers to aid in the diagnosis of breast cancer. Paweletz CP,Trock B,PennanenM,et al. Dis Markers,9 . 2001
[2] PetrieoinⅢ,proteomic patterns of nipple aspirate fluids obtained by SELDI-TOF:potential for new biomarkers to aid in the diagnosis of breast cancer. Paweletz CP,Trock B,PennanenM,et al. Dis Markers,9 . 2001
[3] Biomedical informaties for proteomies. Boguski MS,McIntosh MW. Nature . 2003
[4] Serum protein fingerprinting coupled with a pattern matching algorithm distinguishes prostate cancer from benign prostate hyperplasia and healthy men. Adam B,Qu Y,Davis JW,et al. Cancer Research . 2002
[5] Multiple mechanisms for Pitx-1 transactivation of a luteinizing homn one subunet gene. Spencer D,Robson L,purdy D,et al. Biological Chemistry . 2002
[6] DNA affinity capture and protein profiling by SELDI-TOF mass spectromeltry; effect of DNA methylation. Bane TK,Leblanc JF,Lee TD,et al. Nucleic Acids Research . 2002
[7] Contribution of human alpha-defensin 1,2,and 3 to the anti-HIV-1 activity of CD8 antiviral factor. Zhang L,Yu W,He T,et al. Science . 2002
[8] Surum protein fingerprinting coupled with a pattern matching algorithm distinguishes prostate cancer from benign prostate hyperplasia and healthy men. Adam B,QU Y,Davis JW,et al. Cancer Research . 2002
[9] Surface enhanced laser desorption-ionization retentate chromatography mass spectrometry(SELDI-RC-MS);a new method for rapid development of process chromatography conditions J Chromatogr B Analyt Technol Biomed. Weinberger SR,Boschetti E,Santambien P,et al. Life Sciences . 2002
[10] New technologies for the identification of markers for early detection of ovarian cancer. Bandera CA,Ye B,Mok SC. Cart Opin Obstet Gynecol . 2003

← 1 →