小鼠巨噬细胞膜快速超极化和凋亡

被引:4
作者
黄行许
张淑春
乔东访
鲍永耀
朴英杰
黄有国
hotmail.com
机构
[1] 生物大分子国家重点实验室,中国科学院生物物理研究所!北京,第一军医大学中心实验室!广州,第一军医大学中心实验室!广州,第一军医大学中心实验室!广州,第一军医大学中心实验室!广州,生物大分子国家重点实验室,中国科学院生物物理研究所!北京
关键词
膜电位; 超极化; 凋亡; 巨噬细胞; 地塞米松;
D O I
10.13865/j.cnki.cjbmb.2000.03.023
中图分类号
Q25 [细胞生理学];
学科分类号
071009 ; 090102 ;
摘要
利用激光扫描共聚焦显微技术、流式细胞术、TUNEL染色技术、FRAP技术等对大剂量地塞米松诱导小鼠腹腔巨噬细胞凋亡过程中膜通透性、膜脂流动性、膜电位等膜生物物理性状改变进行了研究 .结果显示 ,大剂量地塞米松诱导小鼠腹腔巨噬细胞快速凋亡 .凋亡巨噬细胞膜脂流动性升高 ,尤为显著的是 ,膜电位快速超极化 ,胞浆游离 Ca2 + 加速超极化 .结果表明 ,细胞膜电位变化与巨噬细胞这一非兴奋细胞凋亡密切相关
引用
收藏
页码:403 / 407
页数:5
相关论文
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