6种染色方法对黄瓜霜霉病菌不同发育阶段的染色效果比较

被引：13

作者：

朱书生

刘西莉

刘鹏飞

范洁茹

袁善奎

机构：

[1] 中国农业大学农学与生物技术学院

来源：

植物病理学报 | 2006年 / 01期

关键词：

荧光染色; 组织学观察; 真菌原位检测;

D O I：

10.13926/j.cnki.apps.2006.01.013

中图分类号：

S436.421 [黄瓜病虫害];

学科分类号：

摘要：

比较了Calcofluor、苯胺蓝、荧光素钠3种荧光染色方法和曲利苯兰酒精-乳酚酸、考马斯亮兰和苯胺蓝组织透明染色法对黄瓜霜霉病菌不同生长发育阶段的染色效果。结果表明,在一定的染液浓度和pH值条件下,3种荧光染色方法均可进行孢子囊、休止孢、芽管、附着胞、孢囊梗等寄主组织外部菌体的原位染色,其中Calcofluor染色方法更稳定、简捷和易于观察。曲利苯兰组织透明染色法能观察黄瓜霜霉病菌的整个生长发育阶段,但该法更适宜胞间菌丝及吸器的染色。

引用

页码：86 / 90

页数：5

共 9 条

[1] Identification and characterization of Myxococcus xanthus mutants deficient in calcofluor white binding. Ramaswamys A,Dworkin M,Downard J. Journal of Bacteriology . 1997
[2] Ultrastructure of Pseudoperonospora cubensis in muskmelon genotypes susceptible and resistant to downy mildew. Cohen Y,Eyal J. Physiological and Molecular Plant Pathology . 1989
[3] An arabidosis mutant with enhanced resistance to powdery mildew. Frye C A,Innes R W. The Plant Cell . 1998
[4] Radial F-actin arrays precede new hypha formation in Saprolegnia: implications for establishing polar growth and regulating tip morphogenesis. Bachewich C,Brent H I. Journal of Cell Science . 1998
[5] A Fluorescent technique for studying growth of Peronospora tabacina on leaf surfaces. Cohen Y,Michael DC. Phytopathology . 1987
[6] Cytological aspects of events occurring after the formation of primary haustoria in barley leaves infected with powdery mildew. Tosa Y,Shishiyama J. Canadian Journal of Botany . 1984
[7] MesA, a novel fungal protein required for the stabilization of polarity axes in Aspergillus nidulans. Pearson C L,Xu K M,Sharpless K E,et al. Molecular Biology . 2004
[8] Methods in cell physiology. Stadelmann E J,Kinzel H. . 1972
[9] The CDC42 homolog of the dimorphic fungus Penicillium mameffei is required for correct cell polarization during growth but not development. Boyce K J,Hynes M J,Rianopoulos A. Journal of Bacteriology . 2001

← 1 →