PEG/(NH4)2SO4双水相体系在加杨叶总黄酮萃取分离中的应用

被引:18
作者
石慧
陈媛梅
机构
[1] 北京林业大学
关键词
双水相体系; 加杨叶总黄酮; 紫外分光光度法;
D O I
10.13241/j.cnki.pmb.2008.05.022
中图分类号
R284 [中药化学];
学科分类号
摘要
目的:寻找加杨叶粗提液中的总黄酮的有效方法。方法:利用双水相体系萃取分离、紫外分光光度法直接测定。结果:萃取分离加杨叶总黄酮的最佳双水相体系是25%PEG 400与12%(NH4)2SO4,最佳萃取条件为:pH=9,NaCl的添加量为3%,粗提液3 mL,温度25℃。结论:该方法的相对标准偏差(RSD)(?)0.28%(n=5),具有良好的精密度和选择性,为黄酮类化合物萃取分离的一种有效方法。
引用
收藏
页码:854 / 857
页数:4
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