“失血加LPS”大鼠肺脏IκBα和TLR4基因表达及参麦的肺脏保护作用

被引：14

作者：

袁兆新 ^{[1
]}

刘潇 ^{[1
]}

季东平 ^{[1
]}

张旭 ^{[2
]}

王志 ^{[1
]}

李扬 ^{[1
]}

赵雪俭 ^{[1
]}

孙连坤 ^{[1
]}

机构：

[1] 吉林大学基础医学院病理生理教研室

[2] 长春医学高等专科学校

来源：

中国病理生理杂志 | 2006年 / 04期

关键词：

中草药; 地塞米松; 脂多糖类; 受体,Toll样; 核因子-κB抑制蛋白α; 肿瘤坏死因子;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

摘要：

目的:探讨参麦注射液的抗休克效果及其作用机制。方法:大鼠随机分为单纯失血性休克(HS)组、失血加脂多糖(HS+LPS)组、地塞米松(HS+LPS+Dex)组和参麦注射液(HS+LPS+SM)组。HS+LPS组大鼠模型复制:首先,大鼠放血至MAP40mmHg,维持10min(失血性休克),然后舌下静脉注射LPS(1·5mg/kg)。4h后留取肺脏组织,RT-PCR法测定IκBα和TLR4mRNA表达、ELISA法测TNF-α含量,并进行肺脏组织电镜观察。结果:HS+LPS+SM组TLR4mRNA表达明显低于HS+LPS组(P<0·05);HS+LPS+SM组IκBαmRNA表达明显高于HS+LPS组(P<0·05);HS+LPS+SM组肺脏组织匀浆TNF-α含量明显低于HS+LPS组(P<0·05);HS+LPS+SM组肺组织病理损伤显著轻于HS+LPS组。结论:参麦注射液能够下调肺脏组织中TLR4mRNA表达,同时上调IκBα的表达,进而抑制促炎介质TNF-α释放,提示参麦注射液可能通过调节NF-κB信号转导途径抑制炎症反应而对机体细胞起保护作用。

引用

页码：730 / 733

页数：4

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