自发性高血压大鼠心脏肥大过程中心肌细胞凋亡与增殖

被引：4

作者：

江立生

方宁远

高天

陆惠华

机构：

[1] 上海第二医科大学附属仁济医院老年病科

[2] 上海第二医科大学附属仁济医院老年病科上海

[3] 上海

来源：

高血压杂志 | 2003年 / 03期

关键词：

凋亡; 增殖; 高血压; 肥大; SHR;

D O I：

10.16439/j.cnki.1673-7245.2003.03.024

中图分类号：

R544.1 [高血压];

学科分类号：

1002 ; 100201 ;

摘要：

目的　观察不同周龄自发性高血压大鼠 (SHR)左心室心肌细胞凋亡与增殖的变化 ,探讨心肌细胞凋亡与增殖在SHR心脏肥大过程中的作用。方法　心脏肥厚指数 (Cardiachypertrophicindex ,CHI)按心脏重量 (mg)与体重 (g)的比值计算 ;末端脱氧核苷酸转移酶介导的dUTP末端标记法 (TUNEL)和透射电镜技术检测SHR心肌细胞凋亡 ;免疫组化技术检测心肌细胞增殖核抗原 (proliferatingcellnuclearantigen ,PCNA)。结果　 (1)与同周龄组WKY相比 ,12w、2 4wSHR心脏肥厚指数显著升高 (P <0 0 1) ,SHR心脏肥厚指数随周龄增加表现出增大的趋势 (SHR16vsSHR12 ,P>0 0 5 ;SHR2 0 vsSHR12 ,P <0 0 1;SHR2 0 vsSHR16,0 0 5 0 1)。 (2 ) 12周龄SHR与同周龄WKY相比 ,左心室心肌细胞凋亡指数 (APOI)无显著增加 (P >0 0 5 ) ,2 4周龄SHR左心室心肌细胞凋亡指数显著低于同周龄WKY(P <0 0 5 ) ;从 12周龄到 2 0周龄 ,SHR左心室心肌细胞APOI逐渐增加 (SHR16vsSHR12 ,0 0 5 <P <0 1;SHR 2 0vsSHR12 ,P <0 0 1) ,到 2 0周龄达到高峰 ,2 0周龄以后 ,SHR左心室心肌细胞APOI显著降低 (SHR2 4vsSHR2 0 ,P <0 0 1)。 (3) 12w、2 4wSHR与同周龄组WKY相比 ,左心室心肌细胞PCNA

引用

页码：84 / 87+106-107

页数：6

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