芹菜非特异性脂转移蛋白基因的克隆与表达分析

被引：6

作者：

蒋倩

王枫

侯喜林

王镇

李梦瑶

马静

刘梦叠

熊爱生

机构：

[1] 南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,农业部华东地区园艺作物生物学与种质创制重点实验室

来源：

园艺学报 | 2012年 / 39卷 / 07期

关键词：

芹菜; 脂转移蛋白; 基因克隆; 实时定量PCR; 基因表达;

D O I：

10.16420/j.issn.0513-353x.2012.07.014

中图分类号：

S636.3 [芹菜];

学科分类号：

摘要：

以芹菜(Apium graveolens)‘六合黄心芹’、‘津南实芹’和‘美国西芹’为试验材料,采用RT-PCR技术分别获得其cDNA序列。序列分析表明:来源于3个芹菜品种的非特异性脂转移蛋白(Non-specific lipid transfer protein,nsLTP)基因核苷酸序列高度保守,全长357bp,编码118个氨基酸,起始密码子ATG之后含有27个氨基酸残基的信号肽序列,推测其成熟的蛋白含91个氨基酸残基,预测其蛋白质分子量为11.75kD,pI值为9.36。芹菜的nsLTP蛋白主要由α-螺旋和随机卷曲组成。空间结构上分析显示,芹菜nsLTP蛋白中H1区域明显分为H1a和H1b两个亚区域,而模板碧桃中H1区域为一个连续的螺旋结构,存在明显的差异。进化分析显示,芹菜nsLTP与香石竹、大洋洲滨藜等植物的nsLTP相似性较高,在保守位置具有8个半胱氨酸残基。实时定量PCR表达分析表明,该基因主要在芹菜的茎以及茎尖生长活跃中心表达,具有明显的组织特异性。

引用

页码：1293 / 1302

页数：10

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