南方鲇生长激素完整cDNA的克隆及其DNA序列分析

被引:16
作者
宋平
向筑
潘云峰
胡隐昌
胡珈瑞
机构
[1] 武汉大学生命科学学院
[2] 中国水产科学研究院珠江水产研究所
[3] 武汉大学中南医院 武汉
[4] 武汉
[5] 广州
关键词
生长激素; cDNA; RT-PCR; 南方鲇;
D O I
暂无
中图分类号
Q523 [脱氧核糖核酸(DNA)];
学科分类号
071010 ; 081704 ;
摘要
采用RT-PCR法和3'、5'RACE(Rapid amplification of cDNA ends)法从南方鲇脑垂体RNA克隆出生长激素(Growth hormone,GH)cDNA。此cDNA全长1087nt(含 Poly(A)》,其5'端非编码区长52nt、阅读框(Open reading frame,ORF)长603nt、3'端非编码区长415nt。其推导的GH由200个氨基酸组成,其中前24个氨基酸为信号肽部分。氨基酸序列比较表明,南方鲇GH与Pangasianodongigas、Ictaluruspunctatus和Heteropneustes fossilis三种鲇类的同源性分别为97.5%和95.0%,与鲢、鳙、草、鲤和鲫鱼的GH同源性达76.0%以上,而与人的GH(Ⅰ、Ⅱ)同源性最低为31.0%和29.5%.
引用
收藏
页码:272 / 280
页数:9
相关论文
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