奇正消痛贴抑制完全弗氏佐剂引起的炎性痛和神经炎症

被引：7

作者：

周利君

桂文珊

刘先国

机构：

[1] 中山大学疼痛研究中心生理教研室广东省脑功能与脑疾病重点实验室

来源：

中国疼痛医学杂志 | 2016年 / 22卷 / 04期

基金：

广东省自然科学基金;

关键词：

慢性炎性痛; 奇正消痛贴膏; Src-家族激酶; 巨噬细胞; 肿瘤坏死因子; 白介素-10;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的:观察奇正消痛贴膏(qizheng pain-relieving plaster,QZPRP)对慢性炎症痛过程中背根神经节(dorsal root ganglion,DRG)和脊髓背角免疫活动异常的影响。方法:成年SD大鼠足跖注射完全弗氏佐剂(complete freund's adjuvant,CFA)建立炎性痛模型。建模后3 d患侧足跖分别包扎QZPRP。用up-down方法和Plantar test分别检测机械痛敏和热痛敏。用ELISA,免疫荧光法和Western blot检测免疫活动。结果:足底使用QZPRP可明显抑制CFA引起的机械痛敏和热痛敏,并促进水肿的恢复,而单纯使用溶剂和药粉无效。QZPRP抑制CFA引起的DRG巨噬细胞浸润、脊髓背角小胶质细胞和星形胶质细胞的激活、DRG和脊髓背角TNF-α上调和SFKs磷酸化。注射CFA上调DRG和脊髓背角的IL-10,QZPRP使其进一步升高。结论:皮肤施加QZPRP可通过抑制痛觉通路的异常免疫活动发挥抗炎镇痛作用,其免疫调节的机制尚有待研究。

引用

页码：250 / 258

页数：9

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