原位PCR技术检测石蜡包埋肝组织中HBV DNA

被引：2

作者：

刘玉兰

杜绍财

陶其敏

机构：

[1] 北京医科大学人民医院肝病研究所

来源：

中华微生物学和免疫学杂志 | 1995年 / 02期

关键词：

聚合酶链反应(PCR); 原位聚合酶链反应(ISPC要R); 原位杂交;

D O I：

暂无

中图分类号：

R446.5 [微生物学检验];

学科分类号：

100208 ;

摘要：

采用原位PCR技术对22例石蜡包埋肝组织中HBV DNA进行检测,并与提取组织DNA PCR技术及原位杂交技术进行比较。结果表明,22例标本经原位PCR检测有9例为阳性;经提取组织DNA PCR检测有7例阳性,两者敏感性相近,符合率达80％;经原位杂交检测有4例阳性,其敏感性只及原位PCR的44％。提示原位PCR是项快速、敏感及特异性较高的检测技术。

引用

页码：67 / 69+75

页数：4

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