四川泡菜中产γ-氨基丁酸微生物的分离及鉴定

被引:5
作者
董玲 [1 ]
朱宇 [1 ]
姚英政 [1 ]
阙付有 [2 ]
机构
[1] 四川省农业科学院农产品加工研究所
[2] 成都伟晖生物科技有限公司
关键词
γ-氨基丁酸; 短乳杆菌; 16S rDNA; 鉴定;
D O I
暂无
中图分类号
TS255.1 [基础科学];
学科分类号
083203 ;
摘要
为了获得具有产γ-氨基丁酸微生物,从四川传统腌制泡菜中分离到30株乳酸菌和5株酵母菌。通过薄层层析定性检测到2株乳酸菌具有产γ-氨基丁酸能力。两株乳酸菌以2%接种量,30℃发酵72h,利用氨基酸自动分析仪定量检测发酵液,γ-氨基丁酸浓度分别为501.4mg/100g、265.4mg/100g。通过16S rDNA序列分析和生理生化鉴定,确定这2株菌为短乳杆菌(Lactobacillus brevis)。5株酵母菌的发酵液均未检测到γ-氨基丁酸。
引用
收藏
页码:77 / 80
页数:4
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