HPLC-柱后生物活性检测在线联用技术在天然活性成分筛选中的应用

被引：13

作者：

王晓飞

焦海胜

李玉民

机构：

[1] 兰州大学第二医院

来源：

中草药 | 2013年 / 44卷 / 08期

关键词：

HPLC; 生物活性检测; 天然产物; 活性成分; 筛选;

D O I：

暂无

中图分类号：

R284 [中药化学];

学科分类号：

100804 [中药化学];

摘要：

HPLC由于分离度和灵敏度高,其在柱后与生物活性检测联用形成的天然活性成分筛选技术,具有操作简单、耗费低、灵敏度和专属性高等优点,已用于天然产物中抗氧化剂、乙酰胆碱酯酶(AChE)抑制剂、磷酸二酯酶(PDE)抑制剂、血管紧张素转移酶(ACE)抑制剂、细胞色素P450抑制剂等的筛选。综述了HPLC-柱后生物活性检测在线联用技术在天然活性成分筛选研究中的应用,同时对其优点和不足进行了总结。

引用

页码：1047 / 1051

页数：5

共 8 条

[1]

联用技术在自由基清除物筛选中的应用 [J].

王璐 ;

孙莉琼 ;

苏航 ;

戚进 ;

余伯阳 .

中草药, 2012, 43 (05) :1032-1036

[2]

High-performance liquid chromatography–mass spectrometry-based acetylcholinesterase assay for the screening of inhibitors in natural extracts.[J].Camiel F. de Jong;Rico J.E. Derks;Ben Bruyneel;Wilfried Niessen;Hubertus Irth.Journal of Chromatography A.2006, 1

[3]

Development of a triple hyphenated HPLC–radical scavenging detection–DAD–SPE–NMR system for the rapid identification of antioxidants in complex plant extracts.[J].Audrius Pukalskas;Teris A. van Beek;Pieter de Waard.Journal of Chromatography A.2005, 1

[4]

Effect-directed analysis by high-performance liquid chromatography with gas-segmented enzyme inhibition.[J].Susanne Fabel;Reinhard Niessner;Michael G. Weller.Journal of Chromatography A.2005, 1

[5]

Antioxidative activity of Geranium macrorrhizum [J].

Miliauskas, G ;

van Beek, TA ;

Venskutonis, PR ;

Linssen, JPH ;

de Waard, P .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2004, 218 (03) :253-261

[6]

Rapid detection and identification of angiotensin-converting enzyme inhibitors by on-line liquid chromatography–biochemical detection; coupled to electrospray mass spectrometry.[J].Danny A. van Elswijk;Otto Diefenbach;Sonja van der Berg;Hubertus Irth;Ubbo R. Tjaden;Jan van der Greef.Journal of Chromatography A.2003, 1

[7]

High-performance liquid chromatography with on-line coupled UV; mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products.[J].K. Ingkaninan;C.M. de Best;R. van der Heijden;A.J.P. Hofte;B. Karabatak;H. Irth;U.R. Tjaden;J. van der Greef;R. Verpoorte.Journal of Chromatography A.2000, 1

[8]

Spectrophotometric determination of phosphate and silicate by sequential injection using molybdenum blue chemistry [J].

Galhardo, CX ;

Masini, JC .

ANALYTICA CHIMICA ACTA, 2000, 417 (02) :191-200

← 1 →