荧光蛋白研究进展

被引：12

作者：

杨杰

张智红

骆清铭

机构：

[1] 华中科技大学武汉光电国家实验室(筹)BrittonChance生物医学光子学研究中心

来源：

生物物理学报 | 2010年 / 26卷 / 11期

关键词：

荧光蛋白; Stokes位移; 光转换与光活化; 荧光显微成像; 高分辨荧光成像;

D O I：

暂无

中图分类号：

Q51 [蛋白质];

学科分类号：

071010 ; 081704 ;

摘要：

荧光蛋白在生物学众多研究领域中有着广泛的应用,基于荧光蛋白的分子探针和标记方法已成为活细胞或活体内动态成像研究生物大分子或细胞功能的重要工具。本文对现有荧光蛋白的种类和理化特性,及其在生物学研究中的应用进行了综述介绍。重点介绍了近年来荧光蛋白在亮度、Stokes位移、光谱改变等方面的研究进展,介绍了光转换与光活化荧光蛋白及其在超分辨荧光成像技术中的应用。最后对荧光蛋白未来的发展方向进行了展望。

引用

页码：1025 / 1035

页数：11

共 70 条

[1]

Erratum to “Visualization of β-secretase cleavage in living cells using a genetically encoded surface-displayed FRET probe” [Biochem. Biophys. Res. Commun. 362 （2007） 25–30][J] . Jinling Lu,Zhihong Zhang,Jie Yang,Jun Chu,Pengcheng Li,Shaoqun Zeng,Qingming Luo. &nbspBiochemical and Biophysical Research Communications . 2007 (4)

[2]

Bright far-red fluorescent protein for whole-body imaging. Shcherbo D,Mer’’zlyak EM,Chepurnykh TV,et al. Nat Methods . 2007

[3]

Excited state proton transfer in the red fluorescent protein mKeima. Henderson JN,Osborn MF,Koon N,Gepshtein R,Huppert D,Remington SJ. Journal of the American Chemical Society . 2009

[4]

Near-infrared fluorescent proteins. Shcherbo D,Shemiakina II,Ryabova AV,Luker KE,Schmidt BT,Souslova EA,Gorodnicheva TV,Strukova L,Shidlovskiy KM,Britanova OV,Zaraisky AG,Lukyanov KA,Loschenov VB,Luker GD,Chudakov DM. Nat Methods . 2010

[5]

THE GREEN FLUORESCENT PROTEIN[J] . Roger Y. Tsien. &nbspAnnual Review of Biochemistry . 1998

[6] Enhanced sensitivity of FRET-based protease sensors by redesign of the GFP dimerization interface [J].

Vinkenborg, Jan L. ;

Evers, Toon H. ;

Reulen, Sanne W. A. ;

Meijer, E. W. ;

Merkx, Maarten .

CHEMBIOCHEM, 2007, 8 (10) :1119-1121

[7]

GFP Family: Structural Insights into Spectral Tuning[J] . Alexey A. Pakhomov,Vladimir I. Martynov. &nbspChemistry & Biology . 2008 (8)

[8] Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals [J].

Lin, Michael Z. ;

McKeown, Michael R. ;

Ng, Ho-Leung ;

Aguilera, Todd A. ;

Shaner, Nathan C. ;

Campbell, Robert E. ;

Adams, Stephen R. ;

Gross, Larry A. ;

Ma, Wendy ;

Alber, Tom ;

Tsien, Roger Y. .

CHEMISTRY & BIOLOGY, 2009, 16 (11) :1169-1179

[9] Far-Red Fluorescent Protein Excitable with Red Lasers for Flow Cytometry and Superresolution STED Nanoscopy [J].

Morozova, Kateryna S. ;

Piatkevich, Kiryl D. ;

Gould, Travis J. ;

Zhang, Jinghang ;

Bewersdorf, Joerg ;

Verkhusha, Vladislav V. .

BIOPHYSICAL JOURNAL, 2010, 99 (02) :L13-L15

[10]

In vivo photoacoustic tomography of chemicals: high-resolution functional and molecular optical imaging at new depths. Kim C,Favazza C,Wang LV. Chemical Reviews . 2010

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