全血法鸡淋巴细胞转化试验最佳试验条件的研究

被引:27
作者
吴建设
张日俊
周毓平
郑世军
杨汉春
郭玉璞
机构
[1] 中国农业大学动物科技学院
[2] 中国农业大学动物医学院
关键词
肉仔鸡,淋巴细胞转化试验,刺激指数,刀豆素A(ConA),脂多糖(LPS);
D O I
暂无
中图分类号
S831 [鸡];
学科分类号
0905 ;
摘要
通过丝裂原浓度、血液稀释度、灭活犊牛血清浓度和培养时间四个参数的测定,确定了鸡全血淋巴细胞转化试验的最佳培养条件。最佳试验方法如下:采用不含灭活犊牛血清的RP-MI1640培养液,刺激管中ConA浓度为45μg/ml或LPS浓度为25μg/ml,血液稀释度为120,40℃培养56h,在培养结束前16h加3H-胸腺嘧啶核苷(3H-TdR)。本法简单方便,可检测T、B细胞的功能状况及用于鸡病病理发生的动力学研究
引用
收藏
页码:21 / 25
页数:5
相关论文
共 10 条
[1]  
I n vitro assay of mitogen stimulation of avian peripheral lymphocytes. Lee L F. Avian Diseases . 1974
[2]  
Optimal conditions for measurement of blastogenic responses of chickens to concanavalin A in whole blood assays. Talebi A,Torgerson P R. Veterinary Immunology and Immunopathology . 1995
[3]  
Stimulation of chicken lymphocytes by T-and B-cell mitogens. Hovi T,Suni J. Cellular Immunology . 1978
[4]  
Stimulation of chicken lymphocytes in a serum-free medium. Kirchner H,and Oppenheim J J. Cellular Immunology . 1972
[5]  
Chicken lymphocyte stimulation by mitogens: a microassay with whole-blood cultures. Lee L F. Avian Diseases . 1978
[6]  
Development of opitmal conditions for the stimulation of chicken peripheral blood lymphocytes by phytohaemagglutimin ( PHA) in the microculture system. Thiel H J,Burkhardt E. Veterinary Immunology and Immunopathology . 1984
[7]  
Transformation of chicken lymphocytes stimulated by concanavalin A: Response Parameters. Nathanson R M. Canadian Journal of Comparative Medicine . 1982
[8]  
A micromethod for stimulation of chicken lymphocytes in vitro using whole blood. Lassila O,Eskola J. Clinical and Experimental Immunology . 1976
[9]  
Optimum conditions for the chicken lymphocyte transformation test. Barta O,Barta V. Avian Diseases . 1992
[10]  
The influence of erythrocytes on the stimulation of chicken lymphocytes by phytohaemagglutinin. Powell P C. Avian Pathology . 1980