磷脂脂肪酸法分析鹤山针叶林和荷木林的土壤微生物多样性

被引：41

作者：

田倩 ^{[1
,2
]}

夏汉平 ^{[1
]}

周丽霞 ^{[1
]}

机构：

[1] 中国科学院华南植物园中国科学院退化生态系统植被恢复与管理重点实验室

[2] 中国科学院研究生院

来源：

热带亚热带植物学报 | 2011年 / 19卷 / 02期

基金：

国家杰出青年科学基金; 国家自然科学基金重点项目;

关键词：

人工林; 磷脂脂肪酸; 土壤微生物多样性; 土层;

D O I：

暂无

中图分类号：

S154.36 [土壤微生物生态学];

学科分类号：

090301 [土壤学];

摘要：

用磷脂脂肪酸法(PLFA)分析广东鹤山两种人工林——针叶林和荷木林不同土层(0～20 cm、20～40 cm、40～60 cm的)土壤微生物群落结构。结果表明,两种人工林的磷脂脂肪酸总量(PLFAs总量)、细菌特征脂肪酸(细菌PLFAs)、真菌特征脂肪酸(真菌PLFAs),以及革兰氏阳性菌和革兰氏阴性菌特征脂肪酸的含量都是0～20 cm土层最高;荷木林的PLFAs总量高于针叶林,但这种差异随土壤深度增加而递减,且与土壤有机碳、全氮含量呈正相关。在相同土层,细菌PLFAs含量均显著高于真菌PLFAs含量。主成分分析表明,土壤微生物多样性及其丰富度受林型和土层综合作用的影响;单一的PLFAs中,i13:0、a13:0、17:0、cy17:0和16:0等脂肪酸对第一主成分的贡献较大;i16:0、cy19:0、18:1ω9 c、i15:0、18:2ω6 c和a17:0等脂肪酸对第二主成分贡献较大。

引用

页码：97 / 104

页数：8

共 23 条

[1]

Trophic transfer of fatty acids from gut microbiota to the earthworm Lumbricus terrestris L [J].

Sampedro, Luis ;

Jeannotte, Richard ;

Whalen, Joann K. .

SOIL BIOLOGY & BIOCHEMISTRY, 2006, 38 (08) :2188-2198

[2]

Plant diversity, soil microbial communities, and ecosystem function: Are there any links? [J].

Zak, DR ;

Holmes, WE ;

White, DC ;

Peacock, AD ;

Tilman, D .

ECOLOGY, 2003, 84 (08) :2042-2050

[3]

The use of phospholipid fatty acids (PL-FA) in the determination of rhizosphere specific microbial communities (RSMC) of two wheat cultivars.[J].Hanan G. Diab El Arab;Vivian Vilich;Richard A. Sikora.Plant and Soil.2001, 2

[4]

Microbial Biomass and Community Structure in a Sequence of Soils with Increasing Fertility and Changing Land Use.[J].H. Yao;Z. He;M. J. Wilson;C. D. Campbell.Department of Soil Science and Agricultural Chemistry; Zhejiang University (Huajiachi Campus); Hangzhou 310029; China;Soil Science Group; Macaulay Land Use Research Institute; Craigiebuckler; Aberdeen AB15 8QH; UK.2000, 3

[5]

Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis..[J].Antonio Gelsomino;Anneke C Keijzer-Wolters;Giovanni Cacco;Jan Dirk van Elsas.Journal of Microbiological Methods.1999, 1

[6]

Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review [J].

Zelles, L .

BIOLOGY AND FERTILITY OF SOILS, 1999, 29 (02) :111-129

[7]

Determinants of soil microbial communities: Effects of agricultural management, season, and soil type on phospholipid fatty acid profiles [J].

Bossio, DA ;

Scow, KM ;

Gunapala, N ;

Graham, KJ .

MICROBIAL ECOLOGY, 1998, 36 (01) :1-12

[8]

Influence of plant development; cultivar and soil type on microbial colonization of maize roots.[J].Luigi Chiarini;Annamaria Bevivino;Claudia Dalmastri;Carlo Nacamulli;Silvia Tabacchioni.Applied Soil Ecology.1998, 1

[9]

Impacts of Carbon and Flooding on Soil Microbial Communities: Phospholipid Fatty Acid Profiles and Substrate Utilization Patterns.[J].D.A. Bossio;K.M. Scow.Department of Land Air and Water Resources; University of California; Davis; CA 95616; USA.1998, 3-4

[10]

Soil Microbial Communities Beneath Populus Grandidentata Grown Under Elevated Atmospheric CO<sub>2</sub>.[J].Donald R. Zak;David B. Ringelberg;Kurt S. Pregitzer;Diana L. Randlett;David C. White;Peter S. Curtis.Ecological Applications.1996, 1

← 1 2 3 →