荧光假单胞菌、沙门氏菌和单增李斯特菌多重PCR检测方法的建立

被引：22

作者：

胡冰雪 ^{[1
]}

舒沿沿 ^{[1
]}

潘道东 ^{[1
,2
]}

曾小群 ^{[1
]}

吴振 ^{[1
]}

机构：

[1] 不详

[2] 宁波大学浙江省动物蛋白食品精深加工技术重点实验室

[3] 不详

[4] 南京师范大学国家乳品加工技术研发分中心

[5] 不详

来源：

食品科学 | 2016年 / 20期

关键词：

多重聚合酶链式反应; 检测方法; 荧光假单胞菌; 沙门氏菌; 单增李斯特菌;

D O I：

暂无

中图分类号：

R440 []; R155.5 [食品卫生与检验];

学科分类号：

摘要：

针对肉制品中易污染的荧光假单胞菌、沙门氏菌和单增李斯特菌等有害微生物,通过3种标准菌株及肉制品建立多重聚合酶链式反应方法,实现肉制品中这3种菌的同时、快速检测。利用荧光假单胞菌的gyrB基因、沙门氏菌的invA基因和单增李斯特菌的hly A基因设计3对特异性引物,在确定引物特异性的基础上,对3种标准菌株及在冷却肉上过夜富集后进行灵敏度检测。结果表明,该多重聚合酶链式反应方法对于同时检测这3种有害微生物具有高度的特异性;同时检测这3种菌时,纯菌DNA检测限可达1 pg/μL;将3种菌一起接种到冷却肉中35℃过夜培养后,荧光假单胞菌、沙门氏菌和单增李斯特菌的检测限分别可达到9、5、70 CFU/m L。

引用

页码：209 / 214

页数：6

共 14 条

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