一株中度嗜盐菌Halomonas sp.NY-011的分离与鉴定

被引：6

作者：

熊焰

吴鹏

尤芳芳

阮琨

曹毅

乔代蓉

白林含

机构：

[1] 四川大学生命科学学院

来源：

四川大学学报(自然科学版) | 2008年 / 05期

关键词：

盐单胞菌属; 中度嗜盐菌; 16S rDNA; 系统发育分析; Biolog代谢指纹分析;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

从盐生杜氏藻（Dunaliella salina）培养物内筛选到一株能在广泛NaCl浓度范围（0.5%21%,w/v）和广泛pH值范围（pH5～11）生长的嗜碱耐盐菌NY-011.细胞形态电镜观察和生理生化分析表明,NY-011呈杆状,大小为（0.51～1.53）μm×（1.02～2.55）μm,革兰氏阴性,有荚膜,无鞭毛,不运动,好氧生长,淀粉水解酶呈阴性,接触酶呈阳性.其最适培养条件为:2%NaCl,30℃,起始pH值10.16SrDNA序列比对和构建的系统发育树表明该菌属于盐单胞菌属（Halomonas）.Biolog分析显示,在检测的95种碳源中,该菌仅有10种的代谢指纹能与数据库类型相匹配,且代谢情况和其亲缘关系最近的Halomonas pantelleriensis亦有差异.

引用

页码：1239 / 1244

页数：6

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