B细胞激活因子受体-BCMA基因表达水平定量标准品的构建

被引:2
作者
陈雯
张芹
黄一红
周锦红
王跃国
刘才旺
杨春兰
沈荣春
鞠少卿
机构
[1] 南通大学附属医院检验医学中心
关键词
B细胞成熟抗原; 标准品; 构建;
D O I
暂无
中图分类号
R450 [];
学科分类号
100215 ;
摘要
目的建立一种简便快速BCMA基因表达水平定量标准品的制备方法。方法PCR扩增目的片段,与T载体连接,转化感受态大肠杆菌DH5α,阳性克隆进行测序证实。结果标准品建立的标准曲线有较大的线性范围,批内和批间重复性也较好,且可长期稳定保存。结论使用质粒标准品对样本中BCMA基因表达水平测定是一种可行的办法。
引用
收藏
页码:10 / 12
页数:3
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