Flt3配体促进再生障碍性贫血患者淋巴细胞向TH1表型分化

被引：5

作者：

傅晋翔

施小凤

李军

陈萍

机构：

[1] 苏州大学附属二院

来源：

中华微生物学和免疫学杂志 | 2004年 / 01期

关键词：

再生障碍性贫血; T细胞分化; Flt3配体;

D O I：

暂无

中图分类号：

R556 [贫血病];

学科分类号：

1002 ; 100201 ;

摘要：

目的　探讨Flt3配体 (FL)对再生障碍性贫血 (AA)辅助性T细胞 (TH)分化的影响及与造血功能衰竭的相关性。方法　应用流式细胞仪分析AA患者骨髓及外周血中TH 亚群 ,并观察植物血凝素 (PHA)和白细胞介素 2 (IL 2 )对AA患者TH 亚群影响 ;体外培养研究FL对骨髓和外周血T细胞分化及细胞因子分泌的作用。结果　AA患者骨髓及外周血中TH1细胞比例明显增高 ,且以急性AA更为显著 (P <0 .0 1) ,TH1与TH2细胞比例失衡 ,TH1 TH2比例显著高于正常对照 (2 .1± 0 .8和 1.4± 0 .7,P <0 .0 5) ;AA及正常人淋巴细胞经PHA和IL 2激发后TH1比例并无明显变化 ,而正常人骨髓细胞经与FL共同孵育 10d后 ,培养上清中IFN γ含量增高 ,表型分析提示 ,除TH1细胞比例增加外 ,树突状细胞及自然杀伤细胞阳性率也有明显增加。结论　FL通过树突状细胞诱导淋巴细胞向TH1表型分化 ,是AA造血功能衰竭的原因之一。

引用

页码：28 / 31

页数：4

共 11 条

[1]

Flt3 ligand augmentation of T cell mitogenesis and expansion of type 1 effector memory T cells. Mosley RL,Parajuli P,Pisarev V,et al. International Journal of Immunopharmacology . 2002

[2]

Flt3 ligand and granulocytemarcophage colony-stimulating factor preferentially expand and stimulate different dendrtic and T-cell subsets. Parajuli P,Mosley LR,Pisarev V,et al. Experimental Hematology . 2001

[3]

Expansion of functional NK cells in multiple tissue compartments of mice treated with flt3-ligand: implications for anti-cancer and anti-viral therapy. Shaw SG,Maung AA,Steptoe RJ,et al. J Immunol . 1998

[4]

Immune pathophysiology of aplastic anemia. Maciejewski JP,Risitano AM,Kook H,et al. International Journal of Hematology . 2002

[5]

Effect of interleukin 10 on the hematopoietic progenitor cells from patients with aplastic anemia. Asano Y,Shibata S,Kobayashi S,et al. Stem Cells . 1999

[6]

Flt3 ligand promotes the generation of a distinct CD34(+) human natural killer cell progenitor that responds to interleukin-15. Yu H,Fehniger TA,Fuchshuber P,et al. Blood . 1998

[7]

Dramatic increase in the numbers of functionally mature dendritic cells in flt3 ligand-treated mice: multiple dendritic cell subpopulations identified. Maraskovsky E,Brasel K,Teepe M,et al. The Journal of Experimental Medicine . 1996

[8]

Acquired aplastic anemia. Young NS. Annals of Internal Medicine . 2002

[9]

Oligoclonal and polyclonal CD4 and CD8 lymphocytes in aplastic anemia and paroxysmal nocturnal hemoglobinuria measured by V beta CDR3 spectratyping and flow cytometry. Risitano AM,Kook H,Zeng W,et al. Blood . 2002

[10]

Mechanism of flt3 ligand expression in bone marrow failure: translocation from intracellular stores to the surface to T lymphocytes after chemotherapy- induced suppression of hematopoiesis. Chklovskais E,Jansen W,Nissen C,et al. Blood . 1999

← 1 2 →