Senescence-likechangesinducedbyexpressionofp21Waf1/Cip1inNIH3T3cellline

被引：14

作者：

XI CHEN WEI ZHANG YUN FEI GAO XIAO QIN SU ZHONG HE ZHAI College of Life Sciences Peking University Beijing China The Key Laboratory of Cell Proliferation and Regulation Biology of the Ministry of Education College of Life Sciences ^{[1
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机构：

来源：

Cell Research | 2002年 / Z1期

关键词：

p21Wafl1/Cip1; senescence; inducible expression; cell cycle arrest;

D O I：

暂无

中图分类号：

Q25 [细胞生理学];

学科分类号：

071009 ; 090102 ;

摘要：

<正>P21Waf1/Cip1 is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21Waf1/Cip1 involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidence for a link between p21Waf1/Cip1 and cellular senescence. While in murine cells, the role of p21Waf1/Cip1 is indefinite. We explored this issue using NIH3T3 cells with inducible p21Waf1/Cip1 expression. Induction of p21Waf1/Cip1 triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features, such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed that p21Waf1/Cip1-transduced NIH3T3 cells expressed β-galactosidase activity at pH 6.0, which is known to be a marker of senescence. Our results suggest that p21Waf1/Cip1 can also induce senescence-like changes in murine cells.

引用

页码：229 / 233

页数：5

共 16 条

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