全长cDNA文库的构建和新基因全长cDNA克隆的策略

被引：6

作者：

何东苟

机构：

[1] 中山大学基础医学院寄生虫学教研室广州

来源：

热带医学杂志 | 2003年 / 04期

关键词：

cDNA文库; 全长cDNA; “电子”基因克隆;

D O I：

暂无

中图分类号：

Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

基因全长cDNA克隆是研究基因功能的前提之一。新基因全长cDNA克隆的方法很多,目前比较可行且应用较多的主要是cDNA文库筛选。本文主要介绍了近年来比较常用的全长cDNA文库的构建及全长cDNA的克隆(包括“电子”基因克隆)的策略和方法及其进展。

引用

页码：473 / 476

页数：4

共 31 条

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