反相离子对高效液相色谱法同时测定维生素B1、B2和菸酸含量

被引：6

作者：

吴怀春

机构：

[1] 军队卫生研究所天津

来源：

营养学报 | 1986年 / 03期

关键词：

水溶性维生素; B1; B2; 菸酸; 反相离子对色谱法;

D O I：

10.13325/j.cnki.acta.nutr.sin.1986.03.014

中图分类号：

学科分类号：

摘要：

本文报告反相离子对高效液相色谱法（HPLC）同时测定强化混合蛋白质中的维生素B1、B2和菸酸。色谱柱为C18,流动相是庚烷磺酸盐液与甲醇（60∶40V/V）,以紫外254nm检测。B1、B2和菸酸的最小检出量分别为15,5和6ng。样品分析CV％为4.71,2.6和5.86;回收率为92.21,90.25和92.33％,试样中其他成份对分析干扰很小,是一种筒单,快速,灵敏和精密度较好的测定方法,可作力强化食品和维生素制剂中B1,B2和菸酸分析用。

引用

页码：273 / 278

页数：6

共 6 条

[1]

Method for the extraction and clean up of animal feed for the determination of hposoluble vitamins A, D and E by high-pressure liquid chromatography. Cohen H,et al. . 1978

[2]

High performance liquid chromatographic analysis of B-vitamins in rice and rice products. Toma R B,et al. . 1979

[3]

Simultaneous determination of vitamins B1, B2, B3 and niacinamide in multivitamin pharmaceutical preparations by paired-ion reversed-phase high-pressure liquid chromatography. Kwak R P,et al. . 1981

[4]

Thiamine and riboflavine analysis by high performance liquid chromatography. Kamman J F,et al. . 1980

[5]

Simultaneous determination of thiamin and riboflavin in selected foods by high performance liquid chromatography. Fellman JK,et al. . 1982

[6]

Simultaneous determination of niacin, niacinamide, pyridoxine, thiamin and riboflavin in multivitamin blends by ion-pair high-pressure liquid chromatography. Kirchmeier RL,et al. . 1978

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