基因组编辑技术在水稻功能基因组和遗传改良中的应用

被引：14

作者：

李希陶

刘耀光

机构：

[1] 华南农业大学生命科学学院亚热带农业生物资源保护与利用国家重点实验室

来源：

生命科学 | 2016年 / 28卷 / 10期

基金：

国家重点研发计划;

关键词：

基因组编辑; CRISPR/Cas9; 功能基因组学; 遗传改良; 水稻;

D O I：

10.13376/j.cbls/2016161

中图分类号：

S511 [稻];

学科分类号：

0901 ;

摘要：

基因组编辑技术对植物基因功能研究和作物遗传改良具有巨大的潜在价值。CRISPR/Cas9系统是继锌指核酸酶(ZFNs)和类转录激活效应因子核酸酶(TALENs)系统之后的新一代基因组编辑技术系统,具有操作简单和效率高等优点。概述了CRISPR/Cas9系统的技术特点及其在水稻基因功能研究及遗传改良中的应用,并指出了该系统在植物基因精准编辑中需要突破的关键问题。

引用

页码：1243 / 1249

页数：7

共 17 条

[1] CRISPR/Cas9:A powerful tool for crop genome editing [J].

Gaoyuan Song ;

Meiling Jia ;

Kai Chen ;

Xingchen Kong ;

Bushra Khattak ;

Chuanxiao Xie ;

Aili Li ;

Long Mao .

The Crop Journal, 2016, 4 (02) :75-82

[2]

植物CRISPR/Cas9基因组编辑系统与突变分析[J]. 马兴亮,刘耀光.遗传. 2016(02)

[3]

Engineering Herbicide Resistant Rice Plants through CRISPR/Cas9-mediated Homologous Recombination of the Acetolactate Synthase[J] . Yongwei Sun,Xin Zhang,Chuanyin Wu,Yubing He,Youzhi Ma,Han Hou,Xiuping Guo,Wenming Du,Yunde Zhao,Lanqin Xia.Molecular Plant . 2016

[4] Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System [J].

Zetsche, Bernd ;

Gootenberg, Jonathan S. ;

Abudayyeh, Omar O. ;

Slaymaker, Ian M. ;

Makarova, Kira S. ;

Essletzbichler, Patrick ;

Volz, Sara E. ;

Joung, Julia ;

van der Oost, John ;

Regev, Aviv ;

Koonin, Eugene V. ;

Zhang, Feng .

CELL, 2015, 163 (03) :759-771

[5]

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants[J] . Xingliang Ma,Qunyu Zhang,Qinlong Zhu,Wei Liu,Yan Chen,Rong Qiu,Bin Wang,Zhongfang Yang,Heying Li,Yuru Lin,Yongyao Xie,Rongxin Shen,Shuifu Chen,Zhi Wang,Yuanling Chen,Jingxin Guo,Letian Chen,Xiucai Zhao,Zhicheng Dong,Yao-Guang Liu.Molecular Plant . 2015 (8)

[6] Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products [J].

Ma, Xingliang ;

Chen, Letian ;

Zhu, Qinlong ;

Chen, Yuanling ;

Liu, Yao-Guang .

MOLECULAR PLANT, 2015, 8 (08) :1285-1287

[7]

DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations[J] . Weizhi Liu,Xianrong Xie,Xingliang Ma,Jun Li,Jiehu Chen,Yao-Guang Liu.Molecular Plant . 2015 (9)

[8] Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice [J].

Rongfang Xu ;

Hao Li ;

Ruiying Qin ;

Lu Wang ;

Li Li ;

Pengcheng Wei ;

Jianbo Yang .

Rice, 2014, 7

[9]

RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System[J] . Kabin Xie,Yinong Yang.Molecular Plant . 2013 (6)

[10] Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs [J].

Shan, Qiwei ;

Wang, Yanpeng ;

Chen, Kunling ;

Liang, Zhen ;

Li, Jun ;

Zhang, Yi ;

Zhang, Kang ;

Liu, Jinxing ;

Voytas, Daniel F. ;

Zheng, Xuelian ;

Zhang, Yong ;

Gao, Caixia .

MOLECULAR PLANT, 2013, 6 (04) :1365-1368

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