UPLC-UV测定细辛地上和地下部位中的马兜铃酸A

被引：35

作者：

薛燕 ^{[1
]}

童湘晖 ^{[2
]}

王峰 ^{[3
]}

赵维国 ^{[3
]}

机构：

[1] 中国医学科学院、中国协和医科大学药物研究所

[2] 北京同仁堂科技发展股份有限公司

[3] 沃特世科技(上海)有限公司

来源：

药学学报 | 2008年 / 02期

关键词：

UPLC; 细辛; 马兜铃酸A;

D O I：

暂无

中图分类号：

R284 [中药化学];

学科分类号：

100804 [中药化学];

摘要：

To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC~ TM HSST3 column （50 mm×2.1 mm ID, 1.8 μm） was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL·min~ -1 , the detection wavelength was 251 nm, and the column temperature was 40 ℃. The calibration curve was linear in the range of 18-1 450 ng of aristolochic acid A with correlation coefficient of 0.999 9 and the minimum detective concentration was 18.25 ng·mL~ -1 . The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.

引用

页码：221 / 223

页数：3