CD4单克隆抗体在体内诱导小鼠胸腺细胞凋亡

被引：9

作者：

周禾

殷金珠

段丹霞

尹忆民

机构：

[1] 北京医科大学免疫系

[2] 北京医科大学实验动物部

[3] 北京医科大学分析计算中心

来源：

中华微生物学和免疫学杂志 | 1995年 / 06期

关键词：

凋亡; CD4 McAb; 胸腺细胞;

D O I：

暂无

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

为研究抗CD4 McAb引起胸腺细胞减少的机理,本课题探讨了CD4 McAb诱导胸腺细胞发生凋亡(Apoptosis)的可能性。小鼠注射抗CD4 McAb后,皮质胸腺细胞体积缩小,染色质凝聚;PI染色后经流式细胞计测定,显示大量的亚二倍体细胞(凋亡细胞,Apoptotic cells),与正常小鼠相比P<0.001;片断化DNA的百分比也明显高于正常小鼠,P<0.01。片断化DNA在凝胶电泳上呈现典型的阶梯现象。细胞内钙离子浓度升高。放线菌酮对抗CD4McAb诱导的DNA片断化有一定的抑制作用。上述结果提示抗CD4 McAb促进胸腺细胞凋亡可能是胸腺细胞减少的原因之一。

引用

页码：15 / 20

页数：6

共 10 条

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