去甲斑蝥素通过JAK-STAT3途径诱导结肠癌LS-174T细胞凋亡

被引：18

作者：

张国建 ^{[1
]}

李猛 ^{[1
]}

王天阳 ^{[2
]}

石铖 ^{[3
]}

王晨宇 ^{[4
]}

闫庆辉 ^{[1
]}

机构：

[1] 河北医科大学第二附属医院肛肠外科

[2] 河北医科大学第二附属医院肝胆外科

[3] 河北省中医院脾胃病科

[4] 河北医科大学第二附属医院微创外科

来源：

肿瘤防治研究 | 2014年 / 41卷 / 01期

关键词：

去甲斑蝥素; AO/EB; AnnexinV/PI:Survivin; stat3;

D O I：

暂无

中图分类号：

R735.35 [];

学科分类号：

摘要：

目的探讨去甲斑蝥素(NCTD)诱导结肠癌LS-174T细胞的凋亡效应及其可能分子机制。方法应用MTT法检测NCTD对细胞生长的抑制作用,通过AO/EB染色、透射电子显微镜观察细胞形态及超微结构的变化,AnnexinV/PI染色检测细胞凋亡率,Western blot检测stat3、survivin、Mcl-1、bax蛋白的表达情况。结果去甲斑蝥素对结肠癌LS-174T细胞具有显著的生长抑制作用,并呈时间—剂量依赖性;40μg/ml作用72 h时达到最大抑制率(72.4±3.1)%;AO/EB染色及流式细胞术显示,随着NCTD浓度的增加,细胞凋亡率明显增加;透射电子显微镜观察发现,结肠癌LS-174T细胞出现明显凋亡超微结构变化;Western blot结果显示,stat3、survivin、Mcl-1表达明显下降,bax表达明显上升。结论去甲斑蝥素可以抑制结肠癌LS-174T细胞的生长并促进其凋亡,机制可能是通过抑制stat3途径实现的。

引用

页码：16 / 21

页数：6

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